Angiotensin I Converting Enzyme 2 (ACE2)Activity Fluorescence Assay Kit

Detection principle: The principle of this kit is that ACE2 catalyzes the decomposition of substrates, releasing fluorescent products. The higher the fluorescence value, the higher the ACE2 activity in the sample. The ACE2 enzyme activity in the sample can be calculated by the standard curve. Performance characteristics: Sample type: serum, plasma, animal tissue,cell Sensitivity: 0.028 U/L Detection range: 0.028-10.47 U/L Detection method: Fluorescence method Assay type: Enzyme Activity Assay time: 40 min Precision: Average inter-assay CV: 4.4-11.8%Average intra-assay CV: 1.5-3.7% Other reagents required: Normal saline (0.9% NaCl) Storage: -20? Valid period: 12 months. Protein function: Essential counter-regulatory carboxypeptidase of the renin- angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1- 9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin- 7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). [The UniProt Consortium]