Amplite(TM) Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*

Amplite(TM) Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-11304 1 kit (200 assays) -

3 - 8 business days*

230.00€
 
Xanthine oxidase (XO) is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and... more
Product information "Amplite(TM) Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*"
Xanthine oxidase (XO) is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. It plays an important role in the catabolism of purines. Xanthine oxidase is normally found in liver and jejunum. During severe liver damage, xanthine oxidase is released into blood, so a blood assay for XO is a way to determine if liver damage has happened. Xanthinuria is a rare genetic disorder where the lack of xanthine oxidase leads to high concentration of xanthine in blood and can cause health problems such as renal failure. The Amplite(TM) Xanthine Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of xanthine oxidase activities. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. In the assay, xanthine oxidase catalyzes the oxidation of purine bases, hypoxanthine or xanthine to uric acid and superoxide , which spontaneously degrades to hydrogen peroxide (H2O2). The kit uses our Amplite(TM) Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at 576 nm. With the Amplite(TM) Xanthine Oxidase Assay Kit, we have detected as little as 0.15 mU/mL xanthine oxidase in a 100 µL reaction volume.
Supplier: AAT Bioquest
Supplier-Nr: 11304

Properties

Application: Xanthine oxidase assays
Format: Homog.

Database Information

KEGG ID : K00106 | Find alternatives

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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