Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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LipidLaunch(TM) LNP Apparent pKa Assay Kit (TNS Method)
LipidLaunch(TM) LNP Apparent pKa Assay Kit (TNS Method)

Item number: Cay702680-96

Cayman's LipidLaunch(TM) LNP Apparent pKa Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pKa of formulated LNPs. The assay uses a fluorogenic probe, 6-(p-toluidino)-2-naphthalenesulfonic acid sodium salt (TNS). This probe fluoresces in association with positively...
Application: Formulated LNP apparent pKa measurement, fluorometric
267.00€ *
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Glycerol Colorimetric Assay Kit
Glycerol Colorimetric Assay Kit

Item number: Cay10010755-96

Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The measurement of circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various...
Application: Glycerol detection
308.00€ *
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Creatinine (serum) Colorimetric Assay Kit
Creatinine (serum) Colorimetric Assay Kit

Item number: Cay700460-192

Cayman's Creatinine (serum) Colorimetric Assay can be used to measure creatinine levels in plasma and serum. The assay relies on the Jaffe' reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate.2 The rate of color development is directly proportional to the concentration...
Application: Creatinine measurement
258.00€ *
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Glutathione Reductase Assay Kit
Glutathione Reductase Assay Kit

Item number: Cay703202-96

Glutathione reductase (GR, EC 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to glutathione (GSH). This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection...
Keywords: GR Assay Kit
Application: GR activity detection
352.00€ *
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PAF Acetylhydrolase Assay Kit
PAF Acetylhydrolase Assay Kit

Item number: Cay760901-96

Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2...
Keywords: Lp-PLA2, PAF-AH
Application: PAF Acetylhydrolase activity detection
Species reactivity: human
327.00€ *
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Protein Carbonyl Colorimetric Assay Kit
Protein Carbonyl Colorimetric Assay Kit

Item number: Cay10005020-96

Cayman's Protein Carbonyl Colorimetric Assay Kit utilizes the DNPH reaction to measure the protein carbonyl content in plasma, serum, cell lysates, or tissue homogenates in a convenient 96-well format. The amount of protein-hydrozone produced is quantified spectrophotometrically at an absorbance between 360-385 nm....
Application: Oxidized protein quantification
428.00€ *
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Free Fatty Acid Fluorometric Assay Kit
Free Fatty Acid Fluorometric Assay Kit

Item number: Cay700310-96

The measurement of FFA can be useful in determining metabolic status. Cayman's Free Fatty Acid (FFA) Fluorometric Assay provides a simple, reproducible, and sensitive tool for measuring free fatty acids in plasma, serum, and urine. The FFA Fluorometric Assay utilizes a coupled enzymatic reaction that results in...
Keywords: FFA
Application: Metabolic studies
554.00€ *
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Candida genus qPCR Assay
Candida genus qPCR Assay

Item number: Cay40675-1

The primers and probes in the Candida genus kit are provided in tube 1 as a 20X concentrated working solution. This is a single assay that will detect: Candida albicans, Candida auris, Candida dubliniensis, Candida glabrata, Candida guiliermondii, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida...
Application: C.albicans/auris/dubliniensis/glabrata/guiliermondii/krusei/lusitaniae/parapsilosis/tropicalis det.
387.00€ *
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EvaEZ(TM) Fluorometric DNA Polymerase Activity Assay
EvaEZ(TM) Fluorometric DNA Polymerase Activity Assay

Item number: BOT-29051

EvaEZ(TM) Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen(R) dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. In the presence of DNA polymerase activity,...
711.00€ *
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Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

Item number: BOT-30009-1

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells. The fluorogenic substrate (Ac-DEVD)2-R110 contains two DEVD...
From 79.00€ *
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NucView(R) 488 Caspase 3 Assay Kit, Trial Size
NucView(R) 488 Caspase 3 Assay Kit, Trial Size

Item number: BOT-30029

NucView(R) 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time. The rate of apoptosis typically varies from cell to cell even within the same population. As a result, various apoptotic events or markers...
From 162.00€ *
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CF(R)488A and 7-AAD Apoptosis Assay Kit
CF(R)488A and 7-AAD Apoptosis Assay Kit

Item number: BOT-30060

This kit contains CF(R)488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry or fluorescence microscopy. Phosphatidylserine (PS) is translocated from the inner to the outer surface of apoptotic cells for phagocytic cell...
372.00€ *
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