Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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Amplite(R) Colorimetric Hexokinase Assay Kit
Amplite(R) Colorimetric Hexokinase Assay Kit

Item number: ABD-11319

The Amplite(R) Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol. This kit utilizes a coupled enzyme assay, where glucose is phosphorylated to glucose-6-phosphate and subsequently oxidized to produce NADH. The...
Application: GAPDH activity measurement
632.00€ *
Review
Amplite(R) Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Amplite(R) Colorimetric Glyceraldehyde-3-Phosphate...

Item number: ABD-11321

The Amplite(R) Colorimetric GAPDH Activity Assay Kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalyzed by GAPDH. This reaction results in the formation of a colorimetric product with an absorbance at 450nm, which is directly proportional to the enzymatic...
Application: Hexokinase activity measurement
377.00€ *
Review
Amplite(R) Fluorimetric Lipase Activity Assay Kit
Amplite(R) Fluorimetric Lipase Activity Assay Kit

Item number: ABD-11322

The Amplite(R) Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity. This assay leverages a coupled enzymatic reaction that converts a substrate into a fluorescent product (Ex/Em=540/610 nm), with fluorescence intensity proportional to the lipase activity present...
Application: Fluorimetric lipase activity measurement
632.00€ *
Review
Amplite(R) Fluorimetric DPP4 Activity Assay Kit
Amplite(R) Fluorimetric DPP4 Activity Assay Kit

Item number: ABD-11323

The Amplite(R) Fluorimetric DPP4 Activity Assay Kit provides a simple, quick, and direct protocol for measuring DPP4 Activity. This assay leverages the DPP4-mediated cleavage of a non-fluorescent substrate, resulting in a fluorescent product with Ex/Em = 360/460 nm, respectively. The fluorescence intensity generated...
Application: DPP4 activity measurement, HTS
632.00€ *
Review
Amplite(R) Fluorimetric DPP4 Inhibitor Screening Kit
Amplite(R) Fluorimetric DPP4 Inhibitor Screening Kit

Item number: ABD-11324

The Amplite(R) Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors. DPP4 activity is quantified by the enzymatic cleavage of a specific substrate, resulting in a fluorescent product (Ex/Em = 360/460 nm), which correlates directly with the...
Application: Fluorimetric DPP4 inhibitor screening, HTS
632.00€ *
Review
Amplite(R) Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Amplite(R) Colorimetric Phosphofructokinase (PFK)...

Item number: ABD-11329

The Amplite(R) Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples. This kit is based on a coupled enzyme assay, in which fructose-6-phosphate in the presence of ATP is converted to...
Application: PFK activity measurement
956.00€ *
Review
Cell Navigator(TM) Flow Cytometric Exosome Staining Kit
Cell Navigator(TM) Flow Cytometric Exosome Staining Kit

Item number: ABD-22426

Correction Factor (280 nm) 0.35. Extinction coefficient (cm^-1 M^-1): 73000. Excitation (nm): 491. Emission (nm): 516. Quantum yield 0.92. Exosomes, nanoscale (30-150 nm) membrane-bound vesicles secreted by cells, are central to intercellular communication. They transfer proteins, lipids, and nucleic acids,...
Application: Fluorimetric CD9-positive exosome detection
632.00€ *
Review
Cell Meter(TM) Colorimetric MTS Cell Proliferation Kit
Cell Meter(TM) Colorimetric MTS Cell Proliferation Kit

Item number: ABD-22766

The Cell Meter(TM) Colorimetric MTS Cell Proliferation Kit is a quick and reliable method for quantifying viable cells in proliferation and cytotoxicity assays. This technique relies on the reduction of the MTS tetrazolium compound by living cells, which produces a colored formazan product soluble in cell culture...
Application: Photometric cell proliferation / cell viability / cytotoxicity quantificaton
From 408.00€ *
Review
Cell Counting Kit 8 (CCK-8)
Cell Counting Kit 8 (CCK-8)

Item number: ABD-22772

Cell Counting Kit 8 (CCK-8) is a colorimetric assay used to quantitate cell proliferation, cell viability and cytotoxicity. Product Description CCK-8 utilizes the enzymatic activity of live cells to reduce a colorless compound into an orange dye, formazan. The amount of formazan produced, as determined by absorbance...
Application: Photometric cell proliferation / cell viability / cytotoxicity quantificaton
From 291.00€ *
Review
Amplite(R) Colorimetric Glucose and Sucrose Assay Kit
Amplite(R) Colorimetric Glucose and Sucrose Assay Kit

Item number: ABD-40009

Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and fructose by the enzyme invertase. The...
Application: Photometric glucose / sucrose measurement
632.00€ *
Review
Amplite(R) Fluorimetric Glucose and Sucrose Assay Kit
Amplite(R) Fluorimetric Glucose and Sucrose Assay Kit

Item number: ABD-40010

Excitation (nm): 571. Emission (nm): 584. Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and...
Application: Fluorimetric glucose / sucrose measurement
632.00€ *
Review
Amplite(R) Rapid Colorimetric APC-Maleimide Quantitation Kit
Amplite(R) Rapid Colorimetric APC-Maleimide Quantitation Kit

Item number: ABD-5527

Maleimide-based crosslinking reagents are commonly used for conjugating proteins to other proteins or biomolecules. A significant challenge in maleimide chemistry is the precise quantification of maleimide moieties conjugated to proteins. The Amplite(R) Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates...
Application: Maleimide group quantificaton
291.00€ *
Review
225 from 273 pages