Amplite(R) Fluorimetric Glucose and Sucrose Assay Kit

Excitation (nm): 571. Emission (nm): 584. Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and fructose by the enzyme invertase. The Amplite(R) Fluorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium. This kit is useful for assessing aerobic glycolysis, pyruvate kinase activity, and metabolic profiles in different biological matrices. It is also employed for determining protein and sugar concentrations in food samples, as well as monitoring plasma insulin and glucose levels in murine models. To measure glucose levels, glucose oxidase specifically oxidizes free glucose, producing a fluorimetric product (Ex/Em = 571/584 nm) that is proportional to the glucose present. To measure sucrose levels, invertase is added to the reaction to convert sucrose into free glucose and fructose, allowing for the measurement of the total glucose level. The sucrose level is then calculated by subtracting the free glucose from the total glucose. Platform: Fluorescence microplate reader, Excitation 540, Emission 590, Cutoff 570. Recommended plate: Solid black. Components: Component A: Amplite(R) Red 1 Vial, Component B: Sucrose Assay Buffer 25 mL, Component C: Invertase 1 Vial, Component D: Glucose Enzyme Mix 1 Bottle, Component E: Sucrose Standard 1 Vial, Component F: DMSO 1 Vial.