Amplite(R) Fluorimetric DPP4 Inhibitor Screening Kit

The Amplite(R) Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors. DPP4 activity is quantified by the enzymatic cleavage of a specific substrate, resulting in a fluorescent product (Ex/Em = 360/460 nm), which correlates directly with the enzymatic activity present. This kit includes Sitagliptin, a well-characterized DPP4 inhibitor used in diabetes treatment, as a reference control, facilitating the assessment of inhibitor efficacy. The assay is optimized for high-throughput screening applications. Dipeptidyl peptidase-4 (DPP4), also referred to as CD26, ADCP2, or DPP, is a transmembrane glycoprotein within the prolyl oligopeptidase family. DPP4 functions as a serine exopeptidase, cleaving N-terminal X-proline and X-alanine residues from polypeptides. It is implicated in numerous physiological processes, including glucose metabolism via regulation of glucagon-like peptide-1 (GLP-1), immune modulation through its role as a receptor on various immune cells, signal transduction as a transmembrane protein responsive to growth factors and chemokines, and tumor suppression via immune system interactions. Thus, DPP4 inhibitors are critical in the therapeutic management of type-2 diabetes. Platform: Fluorescence microplate reader, Excitation 360 nm, Emission 460 nm, Cutoff 435 nm. Recommended plate: Solid black or black plate with clear bottom. Components: Component A: DPP4 Assay Buffer 25 mL, Component B: DPP4 Substrate 0.35 mL, Component C: DPP4 Enzyme 20 µL, Component D: DPP4 Inhibitor (Sitagliptin) 50 µL.