Amplite(R) Fluorimetric Lipase Activity Assay Kit

The Amplite(R) Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity. This assay leverages a coupled enzymatic reaction that converts a substrate into a fluorescent product (Ex/Em=540/610 nm), with fluorescence intensity proportional to the lipase activity present in the sample. This kit is suitable for detecting lipases from various biological samples like cells, supernatants, tissue extracts, or serum, and is adaptable for high-throughput screening applications. Lipases belong to a class of enzymes that catalyze the hydrolysis of ester bonds in lipids, such as triglycerides, phospholipids, and cholesterol esters. These enzymes are essential for the digestion, absorption, and metabolism of dietary fats in the body, playing crucial a role in lipid metabolism and cell signaling. Lipases are produced by various organs and tissues, including the pancreas, liver, intestine, and adipose tissue. Accurate measurement of lipase activity is critical in screening and diagnosis of diseases like pancreatitis, cystic fibrosis, celiac disease, Crohn's disease, and hyperlipidemia. Platform: Fluorescence microplate reader, Excitation 540 nm, Emission 610 nm, Cutoff 570 nm. Recommended plate: Solid black, or black plate with clear bottom. Components: Component A: Lipase Assay Buffer 25 mL, Component B: Lipase Substrate Buffer 10 mL, Component C: Lipase Substrate 0.25 mL, Component D: Lipase Standard 1 Vial.