Anti-J Chain

Applications: , Suitable for use in Double Radial Immunodiffusion and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted in 2mm diameter peripheral wells)., Immunoelectrophoresis: 120ul against 2ul serum or equivalent, Optimal dilutions to be determined by the researcher. Precipitin Titer: 1:32 when tested in agar block immunodiffuison titration against a 1% solution of purified J chain in PBS> , Storage and Stability:, Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.