Anti-IgG3, Biotin conjugated

Applications:, Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested. Recommended Dilution: ELISA: 1:8000 to 1:32,000 , Western Blot: 1:1000-1:5000, Immunohistochemistry: 1:500-1:2000, Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.