Anti-IgG, F(abÕ)2, H&L (HRP)

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:200-1:5,000, Western Blot: 1:200-1:5,000, Immunohistochemistry: 1:500-1:5000 , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.