Anti-IgG F(ab')2, Alkaline Phosphatase conjugated

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilution: Capture ELISA: 1:2000-1:15,000. Assayed against 1ug of Guinea Pig IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT., Western Blot: 1:500-1:2500, Immunohistochemistry: 1:200-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.