DAPI Staining Solution (25ug/mL)

DAPI Reagent (25µg/mL) is developed to identify apoptotic and necrotic cells. DAPI Reagent has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using DAPI and Annexin V.