Purification Media, Affinity, Heparin

Heparin Beads are made by coupling 20-35kD size Heparin extracted from Porcine intestinal mucosa (Type I) to Sepharose beads. These beads are useful for isolating heparin-binding protein such as Antithrombin III, lipoproteins, various enzymes and DNA binding proteins. Heparin Beads Specifications: , Matrix: Sepharose CL-4B, Coupling Method: TargetLock , Heparin Density: 0.7-1.2mg/ml of 20-35kD Heparin. Thrombin binding capacity minimum 0.7mg/ml of beads, Mean Bead Size: 40 -165um , Range of pH: 3.0-11.0, Bead Structure: Highly cross-linked spherical agarose, 4% , Max Back Pressure: 0.3MPa, 3 bar, Max. Flow Rates: 4ml/min/cm2 , Recommended Flow Rate: 1-3ml/min/cm2, Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.