Purification Media, Affinity, Divinyl Sulfone (DVS)

Divinyl? Sulfone? (DVS)? Activated? Beads ?are ?rigid ?Sepharose? beads? loaded? with? high? density? of? reactive? groups.? The? matrix? is? suitable? for? binding ?amino ?or ?hydroxyl? groups, ?present? on? biomolecules ?such ?as ?proteins ?and? carbohydrates. ?The ?DVS? activated? beads? are? stable? for ?extended? period ?of ?time? when ?stored ?refrigerated? and ?light-protected.? Coupling? reactions? to? biomolecules? may ?be ?done? between ?pH ?values ?of ?8-10.??? , ? , DVS? Activated ?Beads Characteristics:, Matrix: ?Sepharose?? CL-4B? Activation ?Method:? Divinyl?Sulfone , Binding ?Capacity: ?2-4?mg BSA/?ml.? , Bead ?Size:? 40-165um? , Bead ?Structure:? Highly ?cross-linked ?spherical ?agarose, ?4%? , Max? Back? Pressure:? 0.3MPa ,?3?bar? , Max.?Flow ?Rates: 15?ml/min/cm2? , Recommended?Flow Rate:? 8-12?ml/min/?cm2? , ? , Buffers? required ?for? protein conjugation (using?10ml?of? DVS Activated Beads): , ?Scale? up? or ?down ?all ?values ?when ?conjugating ?more? or ?less ?than? 10?ml ?of? beads?:, DVS? Activated ?Beads:?10ml, Double? distilled ?water ?(100ml). , Neutral ?conjugation ?buffer ?(50ml):? Phosphate ?Buffered ?Saline? (PBS) ?pH ?6-8?? , Conjugation ?buffer? (50ml):? 0.1N ?Sodium? bicarbonate? buffer ?(pH-8-10).? , Storage and Stability: Store gel refrigerated in any desired buffer added with 0.1% (w/v) sodium azide until use. Use within 6 months after receipt.