Ovalbumin (Agarose)

Many antibodies are made to small peptides or haptens that are injected as Ovalbumin-conjugates. Antibodies are produced against the carrier protein (Ovalbumin) and the coupled peptide or hapten. Anti-Ovalbumin antibodies may interfere with some analyses. The Ovalbumin-agarose column can be used to remove the anti- Ovalbumin antibodies using standard antibody purification techniques. Applications: Suitable for use in removing anti-ovalbumin antibodies in antisera. Other applications not tested. Usage: We recommend processing approximately 1-5ml antiserum per 1ml of the beads or it can be scaled up accordingly. Load antiserum diluted 1:5 in PBS to adsorb anti-Ovalbumin at room temperature. Collect unbound fraction containing Ovalbumin-depleted antiserum. It may be necessary to repeat this adsorption if the sample contains high concentrations of ant-Ovalbumin. Regeneration: The column can be regenerated by passing 3ml of 0.1M Glycine buffer, pH 2.5, and then immediately washing with with 10-20 volumes of PBS, pH 7.4. Store column in PBS containing 0.05% azide at 4°C. DO NOT FREEZE the beads at any stage. Storage and Stability: Stored at 4°C. Do not freeze.