Fixative

ICT's Fixative is a formaldehyde solution designed to cross-link intracellular components. It will not interfere with the fluorescence labels used on our fluorescent detection probes like FLICA, Magic Red, and FLISP. Protocol: If fluorescence-stained cells cannot be evaluated immediately after labeling with the fluorescence probes, add Fixative to the cells at a ratio of 1:10. For example, add 100 µL Fixative to 900 µL cells and incubate for at least 15 minutes at RT in the dark. Never add Fixative until the staining and final wash steps have been completed. Fixed cells may be stored on ice or at 4 °C up to 24 hours.