Endogenous Peroxidase Blocking Buffer

Endogenous Peroxidase Blocking Buffer is mainly used to block endogenous peroxidase in tissues or cells during immunohistochemistry (IHC), immunocytochemistry (ICC) and in situ hybridization (ISH). Endogenous peroxidase is widely found in some cells and tissues, including red blood cells, kidney and liver, which will result with a high background and even false positive results when using peroxidase method to detect samples. Therefore, cell or tissue samples should be blocked with appropriate peroxidase blocking solution before staining to eliminate the interference of endogenous peroxidase. The product is 3% H2O2, which is a ready-to-use buffer to block the endogenous hydrogen peroxide of the sample without dilution.