Kataloge von AAT Bioquest
Buccutite™ Crosslinking Technology
Buccutite™ crosslinking technology by AAT Bioquest offers a truly unique and trouble-free method for labeling primary antibodies with another macromolecule, such as an enzyme or phycobiliprotein. By directly labeling a primary antibody with a fluorophore or enzyme tag, it streamlines the immunostaining procedure by eliminating the need for secondary conjugates and the additional incubation and wash steps that follow. Buccutite™ labeling kits provide all the essential components needed to label 25 μg to 1 mg of antibody. Conjugation takes 2 hours or less to complete, with minimal batch-to-batch variation, no separation step and 100% recovery of antibody. Released March 2026.
Phycobiliproteins & Tandem Conjugates
Phycobiliproteins are a family of photosynthetic light-harvesting proteins derived from microalgae and cyanobacteria. These proteins contain covalently attached linear tetrapyrrole groups, known as phycobilins, which play a critical role in capturing light energy. Because phycobiliproteins have extremely high fluorescence quantum yields and absorbance coefficients over a wide spectral range, they serve as valuable fluorescent tags in a variety of fluorescence applications, primarily flow cytometry. Phycobiliproteins conjugated to molecules having biological specificity (e.g. immunoglobulin, protein A or streptavidin) are effective tools in fluorescence activated cell sorting (FACS), imaging, immunophenotyping and immunoassay applications. Released March 2026.
Fluorescence Microscopy – Imaging Organelles
Organelles are tiny, specialized subunits that perform specific cellular functions. Typically membrane-bound and sequestered within the cytoplasm of eukaryotic cells, these organelles are key components essential for gene expression, cell signaling, energy production and protein synthesis. Detection, visualization and cellular tracking of such organelles in fluorescence imaging applications are made possible with the aid of organelle-selective dyes. Updated March 2026.
AssayWise Letters 13-1
The new AssayWise Letter by AAT Bioquest is all about labeling. Read more about MitoDNA™ Red, a powerful tool for labeling and detection of mitochondrial DNA, or explore the benefits of siRNA labeling, which enables the visualization, quantification, and tracking of siRNAs within cells and tissues. Suitable for this, AAT offers the Helixyte™ iFluor® Dyes for direct, non-enzymatic labeling of plasmids, DNA, RNA, siRNA and oligonucleotides. In this AWL, you will also discover AAT's FastClick™ reagents, which offer a transformative approach to copper-catalyzed azide-alkyne cycloaddition (CuAAC). Released October 2024.
AssayWise Letters 12-1
Read the most recent AssayWise Letter by our partner AAT Bioquest! Learn more about loop-mediated isothermal amplification (LAMP), one of the newest methods of nucleic acid amplification. If you prefer cell biology, take a look at the articles about intercellular channels, focusing on Gap Junctions, and about the potential of 3D cell culture. And in the final part of the letter, you will find a comprehensive guide to cell health assays in flow cytometry. Released December 2023.
AAT Bioquest Selection Guides
The products made by our partner AAT Bioquest speak for themselves, but sometimes selection can still be a challenge, even for relatively common experimental designs. To make the process easier, AAT has built multiple selection guides for common categories of labeling reagents, including relative brightness comparisons where relevant, and all downloadable and printable for user convenience. Selection guides are available for: Annexin V, Antibody and Protein Labeling, Calcein, DNA Dyes, Flow Cytometry, Live or Dead Kits and Phalloidin. Released August 2023.
PE & APC Tandem Dyes
Tandem dyes are highly effective fluorophores that offer exceptional brightness, making them ideal for detecting populations with lower receptor densities in flow cytometry applications. They are composed of a donor dye and an acceptor dye that are covalently linked together to form a single molecule, allowing for an energy transfer cascade (FRET). AAT Bioquest has developed a series of PerCP, PE and APC tandem dyes optimized for the 488 nm, 561 nm, and 633 nm laser lines. These bright dyes provide superior population resolution of low-expression markers and allow for the distribution of fluorochromes across multiple lasers and a wider spectrum. This reduces spillover and simplifies panel design, making the process more efficient and effective for researchers. Released June 2023.
AssayWise Letters 11-1
DNA extraction is a fundamental method in molecular biology, despite being developed unintentionally. In the latest AssayWise Letter by our partner AAT Bioquest, you will get an overview of DNA extraction methods together with a general protocol for DNA extraction and purification. The AWL also includes comprehensive information on the ReadiUseTM 6-Color human TBNK antibody kit, which is designed to provide both the percentages and absolute counts of the lymphocyte subsets T-cells, B-cells, and NK-cells, using gated laser lines commonly present in flow cytometers. On top, you will find a practical overview of quantitative PCR (qPCR). Released April 2022.