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Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme,...
|Anwendung:||Isolating mouse neutrophils by peritoneal lavage, FC|
When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman's MitoCheck(R) Mitochondrial...
Many lipids have a free carboxylic acid group that typically requires a weak acid mobile phase to ensure retention by HPLC. Unfortunately, the presence of the acid in the mobile phase also suppresses anion formation and often leads to poor electrospray ionization and poor MS sensitivity. Cayman's AMP+ MaxSpec(TM)...
|Schlagworte:||GELB Derivitization Kit|
ab 69,00 €
The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates. Therefore, deproteinization is a necessary step in many procedures prior to analysis of biological samples. AAT Bioquest's...
|Anwendung:||Protein removal, Sample preparation, Deproteinization|
Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman's Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman's Transcription Factor...
|Anwendung:||Nuclear extraction, Isolation of nuclear and cytoplasmic fractions|
Apoptosis is associated with the fragmentation of chromosomal DNA into multiples of the 180 bp nucleosomal unit, known as DNA laddering. This kit is designed to maximize extraction of small fragments of DNA with minimal contamination from intact chromatin. The isolated DNA is separated by electrophoresis and can be...
This kit contains all reagents necessary for simple one-step purification of 8-isoprostane from most biological samples.
|Schlagworte:||8-iso Prostaglandin F2alpha Affinity Purification Kit, iPF2alpha-III Affinity Purification Kit, 8-epi Prostaglandin...|
ab 64,00 €