ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird... mehr erfahren »
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ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

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Human Therapeutic IgG1 ELISA Kit
Human Therapeutic IgG1 ELISA Kit

Artikelnummer: Cay500910-96

Human therapeutic antibodies have become increasingly common components of early drug discovery and development portfolios in the pharmaceutical and biotech industries. As part of preclinical toxicology assessment of these agents, they are routinely tested in non-human primates, primarily in rhesus or cynomolgus...
Schlagworte: Human IgG1 Immunometric EIA Kit
Anwendung: ELISA
719,00 €
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DNA/RNA Oxidative Damage ELISA Kit
DNA/RNA Oxidative Damage ELISA Kit

Artikelnummer: Cay589320-96

DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG),...
Schlagworte: 8-hydroxy-2'-Deoxyguanosine, 8-OH-2dG, 8-OH-DG, DNA/RNA Oxidative Damage (High Sensitivity) EIA Kit
Anwendung: ELISA
ab 619,00 €
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Cyclic AMP ELISA Kit
Cyclic AMP ELISA Kit

Artikelnummer: Cay581001-96

Cayman's cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B0) of 0.1 pmol/ml once samples and standards have been acetylated prior to analysis. This assay has been validated with culture media,...
Schlagworte: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit
Anwendung: ELISA
ab 338,00 €
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Prostaglandin F2alpha ELISA Kit
Prostaglandin F2alpha ELISA Kit

Artikelnummer: Cay516011-96

Prostaglandin F2alpha (PGF2alpha) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2alpha is a potent luteolytic...
Schlagworte: PGF2alpha EIA Kit
Anwendung: ELISA
ab 357,00 €
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Prostaglandin E2 Express ELISA Kit
Prostaglandin E2 Express ELISA Kit

Artikelnummer: Cay500141-96

PGE2 is one of the primary COX products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. Because of its involvement in inflammation, PGE2 is the key indicator of non-steroidal...
Schlagworte: PGE2 Express EIA Kit
Anwendung: ELISA
ab 357,00 €
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Testosterone ELISA Kit
Testosterone ELISA Kit

Artikelnummer: Cay582701-96

Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty....
Schlagworte: Testosterone EIA Kit
Anwendung: ELISA
ab 288,00 €
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Thromboxane B2 ELISA Kit
Thromboxane B2 ELISA Kit

Artikelnummer: Cay501020-96

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short...
Schlagworte: TXB2 EIA Kit
Anwendung: ELISA
ab 357,00 €
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Cyclic GMP ELISA Kit
Cyclic GMP ELISA Kit

Artikelnummer: Cay581021-96

The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was...
Schlagworte: cGMP EIA Kit, Guanosine 3',5'-cyclic mononucleotide, Guanosine 3',5'-cyclic monophosphate, Monosodium-GMP
Anwendung: ELISA
ab 338,00 €
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8-Isoprostane ELISA Kit
8-Isoprostane ELISA Kit

Artikelnummer: Cay516351-96

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the...
Schlagworte: 8-epi Prostaglandin F2alpha, 8-iso Prostaglandin F2alpha, 8-Isoprostane EIA Kit, iPF2alpha-III
Anwendung: ELISA
ab 338,00 €
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Prostaglandin E Metabolite ELISA Kit
Prostaglandin E Metabolite ELISA Kit

Artikelnummer: Cay514531-96

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2...
Schlagworte: PGE Metabolite EIA Kit, PGEM
Anwendung: ELISA
ab 344,00 €
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6-keto Prostaglandin F1alpha ELISA Kit
6-keto Prostaglandin F1alpha ELISA Kit

Artikelnummer: Cay515211-96

Prostacyclin (Prostaglandin I2, PGI2) is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1alpha (t½ = 2-3 minutes), and then quickly converted to the major...
Schlagworte: 6-keto PGF1alpha EIA Kit
Anwendung: ELISA
ab 338,00 €
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Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Artikelnummer: Cay514010-96

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo,...
Schlagworte: PGE2 EIA Kit - Monoclonal
Anwendung: ELISA
ab 344,00 €
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