ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird... mehr erfahren »
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ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

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Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Artikelnummer: Cay514010-96

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo,...
Schlagworte: PGE2 EIA Kit - Monoclonal
Anwendung: ELISA
ab 344,00 €
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Anti-Ovalbumin IgG1 (mouse) ELISA Kit
Anti-Ovalbumin IgG1 (mouse) ELISA Kit

Artikelnummer: Cay500830-96

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate...
Anwendung: ELISA
469,00 €
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Anti-Ovalbumin IgE (mouse) ELISA Kit
Anti-Ovalbumin IgE (mouse) ELISA Kit

Artikelnummer: Cay500840-96

Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate...
Anwendung: ELISA
369,00 €
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TNF-alpha (mouse) ELISA Kit
TNF-alpha (mouse) ELISA Kit

Artikelnummer: Cay500850-96

Tumor necrosis factor-alpha (TNF-alpha) mediates many immune and inflammatory responses including activation and differentiation of monocytes and macrophages, expression of MHC class I and II, and expression of adhesion molecules on endothelial cells. Cayman's TNF-alpha (mouse) ELISA Kit is an immunometric (i.e.,...
Schlagworte: Tnf, NTF, ICD2, ICD1, Tnfa, TNF-a, Cachectin, TNF-alpha
Anwendung: ELISA
438,00 €
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Human Therapeutic IgG1 ELISA Kit
Human Therapeutic IgG1 ELISA Kit

Artikelnummer: Cay500910-96

Human therapeutic antibodies have become increasingly common components of early drug discovery and development portfolios in the pharmaceutical and biotech industries. As part of preclinical toxicology assessment of these agents, they are routinely tested in non-human primates, primarily in rhesus or cynomolgus...
Anwendung: ELISA
719,00 €
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Human Therapeutic IgG4 ELISA Kit
Human Therapeutic IgG4 ELISA Kit

Artikelnummer: Cay500920-96

Human therapeutic antibodies have become increasingly common components of early drug discovery and development portfolios in the pharmaceutical and biotech industries. As part of preclinical toxicology assessment of these agents, they are routinely tested in non-human primates, primarily in rhesus or cynomolgus...
Anwendung: ELISA
Reaktivität: Human
719,00 €
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Interleukin-6 (human) ELISA Kit
Interleukin-6 (human) ELISA Kit

Artikelnummer: Cay501030-96

Interleukin-6 (IL-6) is produced by multiple cell types, including monocytes, macrophages, and T cells and is involved in many defense mechanisms including the immune response, hematopoiesis, bone metabolism, and acute phase reactions. IL-6 promotes the proliferation of activated B-cells, acts as a B-cell...
Schlagworte: CDF, IL6, IL-6, BSF-2, IFNB2, IFN-beta-2, Interleukin-6, Interferon beta-2, Hybridoma growth factor, CTL differentiation...
Anwendung: ELISA
357,00 €
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Aldosterone ELISA Kit
Aldosterone ELISA Kit

Artikelnummer: Cay501090-96

Aldosterone, a steroid hormone secreted by the adrenal cortex, is the principle mineralocorticoid controlling sodium and potassium balance. Serum aldosterone levels in normal upright adult individuals are generally less than 300 pg/ml. Only a fraction of urinary aldosterone is excreted intact, thus urinary excretion...
Schlagworte: Aldosterone ELISA
Anwendung: ELISA
ab 344,00 €
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Myeloperoxidase (human) ELISA Kit
Myeloperoxidase (human) ELISA Kit

Artikelnummer: Cay501410-96

Myeloperoxidase (MPO) is a peroxidase enzyme with roles in innate immunoresponse and various diseases. Cayman's MPO (human) ELISA Kit is a sandwich assay that can be used to measure MPO in plasma and serum without prior sample purification. This assay has been validated using plasma and serum from healthy volunteers...
Schlagworte: MPO (human) EIA Kit
Anwendung: ELISA
469,00 €
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DYKDDDDK-Tag Detection ELISA Kit
DYKDDDDK-Tag Detection ELISA Kit

Artikelnummer: Cay501560-96

Cayman's DYKDDDDK-Tag Detection ELISA Kit provides the ability to rapidly assess the levels of DYKDDDDK-tagged proteins at each stage of the expression and purification process. This permits the user to quickly monitor expression efforts and follow protein loss or enrichment at each purification step. This assay is...
Schlagworte: DYKDDDDK-Tag Detection EIA Kit
Anwendung: ELISA, HTS, Sample Preparation
Reaktivität: Broad
248,00 €
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Citrullinated Histone H3 (Clone 11D3) ELISA Kit
Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Artikelnummer: Cay501620-96

Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore...
Schlagworte: CitH3 (Clone 11D3) EIA Kit
Anwendung: ELISA
619,00 €
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Endothelin ELISA Kit
Endothelin ELISA Kit

Artikelnummer: Cay583151-96

The endothelin peptide family consists of three isoforms, ET-1 (corresponding to the initially isolated and most predominant isoform), ET-2, and ET-3. ET-1 is a 21 amino acid peptide and is one of the most potent vasoconstritors currently known. ET-2 displays similar pharmacology to ET-1, whereas ET-3 is a weak...
Anwendung: ELISA
ab 494,00 €
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