ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einen Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird... mehr erfahren »
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ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einen Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

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Prostaglandin E2 Express ELISA Kit
Prostaglandin E2 Express ELISA Kit

Artikelnummer: Cay500141-96

PGE2 is one of the primary COX products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. Because of its involvement in inflammation, PGE2 is the key indicator of non-steroidal...
Schlagworte: PGE2 Express EIA Kit
Anwendung: ELISA
ab 444,00 €
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Human Therapeutic IgG1 ELISA Kit
Human Therapeutic IgG1 ELISA Kit

Artikelnummer: Cay500910-96

Cayman's Human Therapeutic IgG1 ELISA Kit is an immunometric (i.e. sandwich) assay that can be used for the quantification of human IgG1 in monkey, rat, mouse, or rabbit plasma and serum without prior sample purification. The standard curve spans the range of 3.13-200 ng/ml, with a lower limit of quantification...
Schlagworte: Human IgG1 Immunometric EIA Kit
Anwendung: ELISA
895,00 €
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Thromboxane B2 ELISA Kit
Thromboxane B2 ELISA Kit

Artikelnummer: Cay501020-96

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short...
Schlagworte: TXB2 EIA Kit
Anwendung: ELISA
ab 444,00 €
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Corticosterone ELISA Kit
Corticosterone ELISA Kit

Artikelnummer: Cay501320-96

Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress, therefore, corticosterone can be used as a biomarker of stress. Biologists often measure corticosterone in...
Schlagworte: 11beta,21-DHP, 11beta,21-dihydroxy Progesterone, 17-deoxy Cortisol, Corticosterone EIA Kit
Anwendung: ELISA
ab 383,00 €
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Citrullinated Histone H3 (Clone 11D3) ELISA Kit
Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Artikelnummer: Cay501620-96

Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore...
Schlagworte: CitH3 (Clone 11D3) EIA Kit
Anwendung: ELISA
769,00 €
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Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Artikelnummer: Cay514010-96

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo,...
Schlagworte: PGE2 EIA Kit - Monoclonal
Anwendung: ELISA
ab 428,00 €
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Prostaglandin E Metabolite ELISA Kit
Prostaglandin E Metabolite ELISA Kit

Artikelnummer: Cay514531-96

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2...
Schlagworte: PGE Metabolite EIA Kit, PGEM
Anwendung: ELISA
ab 428,00 €
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6-keto Prostaglandin F1alpha ELISA Kit
6-keto Prostaglandin F1alpha ELISA Kit

Artikelnummer: Cay515211-96

Prostacyclin (Prostaglandin I2, PGI2) is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1alpha (t½ = 2-3 minutes), and then quickly converted to the major...
Schlagworte: 6-keto PGF1alpha EIA Kit
Anwendung: ELISA
ab 423,00 €
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Prostaglandin F2alpha ELISA Kit
Prostaglandin F2alpha ELISA Kit

Artikelnummer: Cay516011-96

Prostaglandin F2alpha (PGF2alpha) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2alpha is a potent luteolytic...
Schlagworte: PGF2alpha EIA Kit
Anwendung: ELISA
ab 444,00 €
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8-Isoprostane ELISA Kit
8-Isoprostane ELISA Kit

Artikelnummer: Cay516351-96

Cayman's 8-Isoprostane ELISA Kit is a competitive assay that can be used for quantification of 8-isoprostane in plasma, urine, and other sample matrices. The assay has a range from 0.8-500 pg/ml and a sensitivity (80% B/B0) of approximately 3 pg/ml.Synonyms: iPF2alpha-III, 8-Isoprostane EIA Kit, 8-epi Prostaglandin...
Schlagworte: 8-epi Prostaglandin F2alpha, 8-iso Prostaglandin F2alpha, 8-Isoprostane EIA Kit, iPF2alpha-III
Anwendung: ELISA
ab 422,00 €
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Cyclic AMP ELISA Kit
Cyclic AMP ELISA Kit

Artikelnummer: Cay581001-96

Cayman's cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B0) of 0.1 pmol/ml once samples and standards have been acetylated prior to analysis. This assay has been validated with culture media,...
Schlagworte: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit
Anwendung: ELISA
ab 422,00 €
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Cyclic GMP ELISA Kit
Cyclic GMP ELISA Kit

Artikelnummer: Cay581021-96

The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was...
Schlagworte: cGMP EIA Kit, Guanosine 3',5'-cyclic mononucleotide, Guanosine 3',5'-cyclic monophosphate, Monosodium-GMP
Anwendung: ELISA
ab 422,00 €
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