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Leupeptin (Ac-Leu-Leu-Arginal-hemisulfate) Artikelnummer: 12136.25
Leupeptin, also known as N-acetyl-L-leucyl-L-leucyl-L-argininal, is a naturally occurring protease inhibitor that can inhibit cysteine, serine and threonine peptidases. It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases,...
| Anwendung: | Inhibitor |
| CAS | 103476-89-7 |
| MW: | 475.6 |
ab 83,00 €
Glycine, ultra pure Artikelnummer: 04943.1
Glycine (abbreviated as Gly or G) is the smallest of the 20 amino acids commonly found in proteins, having a hydrogen substituent as its side-chain. Its codons are GGU, GGC, GGA, GGG of the genetic code. Glycine is often used as a counterion in SDS-PAGE and in buffers for western blotting.
| Anwendung: | Buffer component, Protein stabilization |
| CAS | 56-40-6 |
| MW: | 75.07 |
ab 30,00 €
Isopropyl-beta-D-thiogalacto- pyranoside (IPTG) Artikelnummer: 05684.25
Purity: >99%. Free of dioxane and other solvents. For use im molecular biology. In combination with X-Gal (cat# 02249) it is used to detect lac+ colonies in cloning experiments.
| Anwendung: | Inducer of beta-galactosidase |
| CAS | 367-93-1 |
| MW: | 238.30 |
ab 32,00 €
Dimethylsulfoxide (DMSO) Artikelnummer: 54686.100
DMSO is a polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. DMSO is less toxic than other members of this class, such as dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and HMPA. DMSO is also used in PCR to...
| Anwendung: | Protein stabilization |
| CAS | 67-68-5 |
| MW: | 78.13 |
ab 29,00 €
Dithiothreitol (DTT) Artikelnummer: 04010.100
DTT is an unusually strong reducing agent, because once oxidized, it forms a stable six-membered ring with an internal disulfide bond. The reduction usually does not stop at the mixed-disulfide species because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a...
| Anwendung: | Reducing agent |
| CAS | 3483-12-3 |
| MW: | 154.25 |
ab 40,00 €
Bluo-Gal, pure Artikelnummer: 50375.1
5-Bromo-3-Indolyl-ß-D-Galactopyranoside (Bluo-Gal) is a substrate for the histochemical localization of ß-galactosidase. Bluo-Gal is an alternative to X-Gal, producing a darker blue color.
| Schlagworte: | 5-Bromo-3-indolyl beta-D-galactopyranoside |
| Anwendung: | Beta-Galactosidase substrate |
| CAS | 97753-82-7 |
| MW: | 374.20 |
176,00 €
NADH, disodium salt Artikelnummer: 16132.1
Assay: >85% enzymatically
| Schlagworte: | beta-Nicotinamide-adenine-dinucleotide, reduced Na2-salt |
| CAS | 606-68-8 |
| MW: | 709.4 D |
ab 46,00 €
Thiazolyl blue (MTT) 3-(4,5-Dimethyl-2-thiazolyl)-2... Artikelnummer: 15655.1
MTT, a yellow tetrazole, is reduced to purple formazan in living cells. A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a...
| Anwendung: | Cell viability assay |
| CAS | 298-93-1 |
| MW: | 414.33 |
ab 28,00 €
NADPH, tetrasodium salt Artikelnummer: 16156.100
Nicotinamide adenine dinucleotide phosphate, abbreviated NADP+ or, in older notation, TPN (triphosphopyridine nucleotide), is a coenzyme used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+. NADP+ differs from NAD+ in the...
| Schlagworte: | beta-Nicotinamide-adenine-dinucleotide phosphate, reduced Na4-salt |
| CAS | 53-57-6 |
| MW: | 833.36 D |
ab 72,00 €
Ionomycin (calcium salt) Artikelnummer: Cay11932-1
Ionomycin is a calcium ionophore originally isolated from S. conglobatus. It induces intracellular calcium mobilization in X. laevis oocytes when used at a concentration of 5 µM. Ionomycin (5 µM) induces the formation of neutrophil extracellular traps (NETs) in isolated human neutrophils. Ionomycin in combination...
| Schlagworte: | 11,19R,21S-trihydroxy-4R,6S,8S,12R,14R,18R,20S-heptamethyl-22-[octahydro-5'S-[1R-hydroxyethyl]-2S,5'-dimethyl[2,2'R-bifura... |
| Anwendung: | Calcium ionophore |
| CAS | 56092-82-1 |
| MW: | 747.1 D |
ab 32,00 €
Amphotericin B (Fungizone) Solution, 100X Artikelnummer: A2230.100
Amphotericin B is a polyene antifungal agent for yeast, mold and mycoplasma. It was first isolated by Gold, et a., from Streptomyces nodosus in 1955. It is an amphoteric compound composed of a hydrophilic polyhydroxyl chain along one side and a lipophilic polyene hydrocarbon chain on the other. Amphotericin B is...
| CAS | 1397-89-3 |
| MW: | 924.09 D |
ab 70,00 €
Y-27632 (hydrochloride) Artikelnummer: Cay10005583-10
The ROCK family of Rho-associated serine-threonine protein kinases is known to play an important role in Rho-mediated cell adhesion and smooth muscle contraction. Y-27632 is a potent, ATP-competitive inhibitor of ROCKs including p160ROCK (Ki = 140 nM) and ROCK-II (IC50 = 800 nM). It also inhibits PRK2 with an IC50...
| Schlagworte: | 4-[(1R)-1-aminoethyl]-N-4-pyridinyl-trans-cyclohexanecarboxamide, dihydrochloride |
| Anwendung: | ROCK inhibitor |
| CAS | 129830-38-2 |
| MW: | 320.3 D |
ab 57,00 €