Zellbasierte Assays

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Glycolysis Cell-Based Assay Kit
Glycolysis Cell-Based Assay Kit

Artikelnummer: Cay600450-1

Cayman's Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between...
Anwendung: Extracellular L-lactate detection
Format: Homog.
313,00 €
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Glucose Uptake Cell-Based Assay Kit
Glucose Uptake Cell-Based Assay Kit

Artikelnummer: Cay600470-1

Glucose metabolism is a primary source of energy and biomaterials for the maintenance of cell homeostasis. Extra glucose is stored in the muscles and liver as glycogen which is hydrolyzed to glucose and released into the blood when needed. The rate of glucose uptake in cells is dynamic and tightly regulated by hormones and/or growth factors including insulin. Cancer cells...
Anwendung: Fluorescence
313,00 €
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WST-1 Cell Proliferation Assay Kit
WST-1 Cell Proliferation Assay Kit

Artikelnummer: Cay10008883-96

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. Cayman's WST-1 Proliferation Assay Kit provides an easy to use tool for studying the...
Anwendung: Cell based assays
Format: Homog.
ab 63,00 €
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Lipid Droplets Fluorescence Assay Kit
Lipid Droplets Fluorescence Assay Kit

Artikelnummer: Cay500001-480

Cayman's Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets. Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and...
Anwendung: Fluorescence
Format: Homog.
223,00 €
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Phagocytosis Assay Kit (IgG FITC)
Phagocytosis Assay Kit (IgG FITC)

Artikelnummer: Cay500290-1

Cayman's Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the...
Anwendung: FC
Format: Bead Based
313,00 €
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Hydrogen Peroxide Cell-Based Assay Kit
Hydrogen Peroxide Cell-Based Assay Kit

Artikelnummer: Cay600050-96

Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets. Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP...
Anwendung: Fluorescence
Format: Homog.
ab 102,00 €
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Autophagy/Cytotoxicity Dual Staining Kit
Autophagy/Cytotoxicity Dual Staining Kit

Artikelnummer: Cay600140-1

Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms. Autophagy is a multi-step process that begins...
Anwendung: Fluorescence
Format: Homog.
318,00 €
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HDAC Cell-Based Activity Assay Kit
HDAC Cell-Based Activity Assay Kit

Artikelnummer: Cay600150-96

Nucleosomes, which fold chromosomal DNA, contain two molecules each of the core histones H2A, H2B, H3, and H4. Almost two turns of DNA are wrapped around this octameric core, which represses transcription. The histone amino termini extend from the core, where they can be modified post-translationally by acetylation, phosphorylation, ubiquitination, and methylation, affecting...
Anwendung: HDAC activity modulator studies
Format: Homog.
318,00 €
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Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence)
Secreted Alkaline Phosphatase Reporter Gene...

Artikelnummer: Cay600260-300

Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman's Secreted Alkaline Phosphatase Reporter Gene Assay Kit...
Anwendung: Chemiluminescence
Format: Homog.
287,00 €
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Annexin V FITC Assay Kit
Annexin V FITC Assay Kit

Artikelnummer: Cay600300-100

One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine residues to the outer plasma membrane leaflet allows their detection via their...
Anwendung: Cell based assays, FC, Fluorescence
Format: Homog.
350,00 €
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NAD/NADH Cell-Based Assay Kit
NAD/NADH Cell-Based Assay Kit

Artikelnummer: Cay600480-1

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman's NAD/NADH Cell-Based Assay Kit provides a...
Anwendung: Measure intracellular NAD?
Format: Homog.
369,00 €
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Phagocytosis Assay Kit (IgG PE)
Phagocytosis Assay Kit (IgG PE)

Artikelnummer: Cay600540-1

Cayman's Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the...
Anwendung: FC
Format: Bead Based
344,00 €
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