Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo-Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo-Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien... mehr erfahren »
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Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo-Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo-Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

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NZY5alpha
NZY5alpha

Artikelnummer: NZY-MB00401

NZY5alpha Competent Cells display similar properties to DH5alpha, which are suitable for high efficiency transformation in a wide variety of applications. The phi80dlacZ?M15 marker provides alpha-complementation of the beta-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white...
Anwendung: Blunt-end cloning, Ligation Independent cloning (LIC), TA cloning
ab 89,00 €
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NZYStar
NZYStar

Artikelnummer: NZY-MB00501

NZYStar Competent Cells are suitable for general cloning protocols and for the construction of gene banks or the generation of cDNA libraries using plasmid-derived vectors. Tetracycline ensures that the selectable F' containing lacZalpha M15 is maintained and thus eliminates the background of non-recombinant white...
Anwendung: Blunt-end cloning, Ligation Independent cloning (LIC), Mutagenesis, TA cloning
ab 99,00 €
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Firefly Luciferase Molm13 Cell Line
Firefly Luciferase Molm13 Cell Line

Artikelnummer: BPS-78372

Recombinant Molm13 cells constitutively expressing the firefly (Photinus pyralis) luciferase reporter gene under the control of a CMV promoter.
Schlagworte: Luciferase, Luciferin 4-monooxygenase,
Anwendung: CAR-T / NK co-culture killing assay internal control, bioluminescence Imaging
5.450,00 €
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LAIR1 CHO Cell Line
LAIR1 CHO Cell Line

Artikelnummer: BPS-78913

LAIR1 CHO Cell Line is a clonal CHO cell line stably expressing full-length human LAIR1 (leukocyte-associated immunoglobulin-like receptor 1) (NM_002287.6). Surface expression of LAIR1 was confirmed by flow cytometry. This stable clonal cell line was selected for high levels of LAIR1 expression compared to the...
Schlagworte: LAIR1, CD305, hLAIR1, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1,
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
6.020,00 €
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VSIG4 CHO Cell Line
VSIG4 CHO Cell Line

Artikelnummer: BPS-78914

VSIG4 CHO Cell Line is a clonal CHO cell line stably expressing full-length human VSIG4 (V-set and immunoglobulin domain containing 4) (NM_007268.3). Surface expression of VSIG4 was confirmed by flow cytometry. This stable clonal cell line was selected for high levels of VSIG4 expression compared to the parental...
Schlagworte: CRIg, VSIG4, Protein Z39Ig, V-set and immunoglobulin domain-containing protein 4,
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
6.020,00 €
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NKp46 CHO Cell Line (High Expression)
NKp46 CHO Cell Line (High Expression)

Artikelnummer: BPS-78915-H

NKp46 CHO Cell Line is a clonal CHO cell line stably expressing full-length human NKp46 (Natural cytotoxicity triggering receptor 1) (NM_004829). Surface expression of NKp46 was confirmed by flow cytometry. Stable clonal cell lines were selected for different levels of NKp46 expression (low, medium, and high)...
Schlagworte: NCR1, LY94, CD335, NKp46, NK-p46, hNKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, Natural killer cell...
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
3.207,00 €
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NKp46 CHO Cell Line (Low Expression)
NKp46 CHO Cell Line (Low Expression)

Artikelnummer: BPS-78915-L

NKp46 CHO Cell Line is a clonal CHO cell line stably expressing full-length human NKp46 (Natural cytotoxicity triggering receptor 1) (NM_004829). Surface expression of NKp46 was confirmed by flow cytometry. Stable clonal cell lines were selected for different levels of NKp46 expression (low, medium, and high)...
Schlagworte: NCR1, LY94, CD335, NKp46, NK-p46, hNKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, Natural killer cell...
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
3.207,00 €
Bewerten
NKp46 CHO Cell Line (Medium Expression)
NKp46 CHO Cell Line (Medium Expression)

Artikelnummer: BPS-78915-M

NKp46 CHO Cell Line is a clonal CHO cell line stably expressing full-length human NKp46 (Natural cytotoxicity triggering receptor 1) (NM_004829). Surface expression of NKp46 was confirmed by flow cytometry. Stable clonal cell lines were selected for different levels of NKp46 expression (low, medium, and high)...
Schlagworte: NCR1, LY94, CD335, NKp46, NK-p46, hNKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, Natural killer cell...
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
3.207,00 €
Bewerten
NLRP3 Knockdown THP-1 Cell Pool
NLRP3 Knockdown THP-1 Cell Pool

Artikelnummer: BPS-82121

NLRP3 Knockdown THP-1 Cell Pool is a cell pool of THP-1 human monocytic cells. This cell pool was generated by using NLRP3 Human shRNA Lentivirus (BPS Bioscience #82122) to create a THP-1 cell pool with stable knockdown of NLRP3 (NLR family pyrin domain containing 3). THP-1 cells are the most commonly used model...
Schlagworte: CLR1.1, Cryopyrin, Caterpiller protein 1.1, PYRIN-containing APAF1-like protein 1, Angiotensin/vasopressin receptor...
Anwendung: NLRP3 knockdown effect on cellular function studies
Spezies-Reaktivität: human
1.817,00 €
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ZsGreen/Luciferase Safe-Harbor HEK293 Cell Line
ZsGreen/Luciferase Safe-Harbor HEK293 Cell Line

Artikelnummer: BPS-82091

Recombinant stable HEK293 cell line constitutively expressing ZsGreen (bright green fluorescent protein derived from Zoanthus sp. reef coral) and firefly luciferase, which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19 using CRISPR/Cas9. Expression of ZsGreen and the luciferase...
Schlagworte: Luciferase, Luciferin 4-monooxygenase,
Anwendung: Cell-based assay control, base cell line model for further genetic modifications of interest
Spezies-Reaktivität: human
2.239,00 €
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Claudin-1 CHO Cell Line
Claudin-1 CHO Cell Line

Artikelnummer: BPS-82097

CHO cell line stably expressing human Claudin-1 (NM_021101.5). Surface expression of Claudin-1 was confirmed by flow cytometry. The stable clonal cell line was selected for its high levels of Claudin-1 expression compared to the parental CHO-K1 cell line.
Schlagworte: CLD1, CLDN1, Claudin-1, Senescence-associated epithelial membrane protein,
Anwendung: Antibody screening/validation, drug discovery, binding assays, ligand screening
Spezies-Reaktivität: human
9.308,00 €
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eGFP/Firefly Luciferase Ramos (RA 1) Cell Line
eGFP/Firefly Luciferase Ramos (RA 1) Cell Line

Artikelnummer: BPS-82149

eGFP/Firefly Luciferase Ramos (RA 1) Cell Line is a Ramos (RA 1) cell line expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.
Schlagworte: Luciferase, Luciferin 4-monooxygenase,
Anwendung: CAR-T / NK co-culture killing assay target cells, In vitro/in vivo bioluminescence imaging
Spezies-Reaktivität: human
3.478,00 €
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