Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien... mehr erfahren »
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Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

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GLP-1R/CRE (Luc) Reporter - HEK293 Recombinant Cell Line
GLP-1R/CRE (Luc) Reporter - HEK293 Recombinant Cell Line

Artikelnummer: BPS-78176

Recombinant HEK293 cells expressing firefly luciferase gene under the control of cAMP response element (CRE) with constitutive expression of human GLP-1R (Glucagon-like peptide 1 receptor, accession number BC113493). GLP-1R, a member of the class B family of G protein-coupled receptors (GPCRs) primarily found in...
Schlagworte: GLP1R, GLP-1R, GLP-1-R, GLP-1 receptor, Glucagon-like peptide 1 receptor,
Anwendung: Agonist screening in cellular context
Reaktivität: Human
10.274,00 €
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Cas9-Expressing MCF7 Cell Pool
Cas9-Expressing MCF7 Cell Pool

Artikelnummer: BPS-78179

Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1,
Anwendung: Quick knock-out cell pool / cell line generation, SgRNA screening
Reaktivität: Streptococcus Pyogenes Serotype M1
998,00 €
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BCMA / CD20 / Firefly Luciferase CHO Cell Line
BCMA / CD20 / Firefly Luciferase CHO Cell Line

Artikelnummer: BPS-78185

Clonal stable CHO cell line constitutively expressing full length human BCMA protein (also known as CD269 or TNFRSF17, Genbank accession #NM_001192) and human CD20 protein (also known as MS4A1 or FMC7, Genbank accession #NM_021950). This cell line was derived from our CHO-K1 Luciferase cells BPS-79725, therefore it...
Schlagworte: BCM, CD269, TNFRSF17, B-cell maturation protein, Tumor necrosis factor receptor superfamily member 17,
Anwendung: CAR-T studies
Reaktivität: Human
11.289,00 €
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CD19 / CD20 / Firefly Luciferase CHO Cell Line
CD19 / CD20 / Firefly Luciferase CHO Cell Line

Artikelnummer: BPS-78186

Clonal stable CHO cell line constitutively expressing full length human CD19 protein (also known as CVID3, Genbank accession #NM_001770) and human CD20 protein (also known as MS4A1 and FMC7, Genbank accession #NM_021950). This cell line was derived from our CHO-K1 Luciferase cells BPS-79725, therefore it also...
Schlagworte: CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, T-cell surface antigen Leu-12, B-lymphocyte surface antigen...
Anwendung: CAR-T studies
Reaktivität: Human
11.289,00 €
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TROP2 - CHO-K1 Recombinant Cell Line
TROP2 - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78099

Recombinant CHO-K1 cells expressing the cell surface glycoprotein TROP2 (Trophoblast cell-surface antigen 2), ref. seq. NM_002353.2.
Schlagworte: GA733-1, TACSTD2, Cell surface glycoprotein Trop-2, Pancreatic carcinoma marker protein GA733-1, Tumor-associated calcium...
Anwendung: Screening, Validating antibodies, CAR-T, Immunotherapy research, Drug discovery
Reaktivität: Human
7.428,00 €
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GPC3 - CHO-K1 Recombinant Cell Line
GPC3 - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78100

Recombinant CHO-K1 cells expressing GPC3 (Glypican 3), a cell surface proteoglycan bearing heparin-sulfate, ref. seq. NM_004484.2.
Schlagworte: OCI5, GPC3
Anwendung: Screening, Validating antibodies, CAR-T, Immunotherapy research, Drug discovery
Reaktivität: Human
7.428,00 €
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Human Mesothelin - CHO-K1 Recombinant Cell Line
Human Mesothelin - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78132

Recombinant CHO-K1 cells expressing cleaved, mature human Mesothelin (MSLN) gene (GenBank Accession #NM_005823.5) under the control of cytomegalovirus (CMV) promoter. This cell line was generated by limited dilution and isolation of individual clones, which were screened based on MSLN expression to obtain a...
Schlagworte: MPF, MSLN
Anwendung: MSLN-expressing target cells, MSLN CAR-T cells co-culture assays, Screening
Reaktivität: Human
7.428,00 €
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Cas9-Expressing HCT116 Cell line - High expression
Cas9-Expressing HCT116 Cell line - High expression

Artikelnummer: BPS-78135-H

This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS-78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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Cas9-Expressing Jurkat Cell line - High expression
Cas9-Expressing Jurkat Cell line - High expression

Artikelnummer: BPS-78136-H

This cell line is a clonal derivative from the Cas9-Expressing Jurkat Cell Pool (BPS-78070). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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Cas9-Expressing Neuro2a Cell line - High expression
Cas9-Expressing Neuro2a Cell line - High expression

Artikelnummer: BPS-78137-H

This cell line is a clonal derivative from the Cas9-Expressing Neuro2a Cell Pool (BPS-78087). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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Human EGFRvIII- CHO K1 Recombinant Cell Line (High or Low Expression)-78145-H
Human EGFRvIII- CHO K1 Recombinant Cell Line (High or Low...

Artikelnummer: BPS-78145-H

Recombinant hEGFRvIII-CHO K1 cell line stably expressing full-length human EGFRvIII receptor (accession number NP_001333870). Surface expression of hEGFRvIII was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hEGFRvIII expression (High and Low) to mimic different...
Schlagworte: ERBB, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase erbB-1
Anwendung: EGFRvIII signaling inhibitor screening, Immuno-oncology research, Drug discovery
Reaktivität: Human
7.428,00 €
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CD38 / BCMA / Firefly Luciferase CHO Recombinant Cell Line
CD38 / BCMA / Firefly Luciferase CHO Recombinant Cell Line

Artikelnummer: BPS-78148

Recombinant clonal stable CHO cell line constitutively expressing full length human CD38 protein (also known as ADPRC1, Genbank accession #NM_001775) and human BCMA protein (B-Cell Maturation Antigen or CD269, GenBank accession #NM_001192). This cell line was derived from our CHO-K1 Luciferase cells (BPS-79725),...
Schlagworte: T10, CD38, ADPRC 1, EC=3.2.2.6, EC=2.4.99.20, cADPr hydrolase 1, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1,...
Anwendung: Validation
Reaktivität: Human
10.274,00 €
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