Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien... mehr erfahren »
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Zellen und Zelllinien

Zu den bedeutendsten Entwicklungen der biologischen Forschung in den letzten 200 Jahren gehört die Einführung der Zellkultur. Mikrobenkulturen von Bakterien und Hefen in sterilen Medien ermöglichen das selektive Züchten monoklonaler Kolonien von Zellen. Aus diesen Kolonien können z.B. kompetente Zellen für Transformationen gewonnen werden. Bei der Anzucht tierischer Zellen in Wachstumsmedien wird zwischen ex vivo Kulturen und immortalisierten Zelllinien unterschieden. Ex vivo Kulturen werden direkt aus Blut oder Organen generiert und besitzen eine begrenzte Lebensdauer. Immortalisierte Zelllinien wie HeLa oder HEK 293T hingegen sind aus Tumorzellen abgeleitet und verfügen über Mutationen, dank derer sie dauerhaft proliferieren können.

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TROP2 - CHO-K1 Recombinant Cell Line
TROP2 - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78099

Recombinant CHO-K1 cells expressing the cell surface glycoprotein TROP2 (Trophoblast cell-surface antigen 2), ref. seq. NM_002353.2.
Schlagworte: GA733-1, TACSTD2, Cell surface glycoprotein Trop-2, Pancreatic carcinoma marker protein GA733-1, Tumor-associated calcium...
Anwendung: Screening, Validating antibodies, CAR-T, Immunotherapy research, Drug discovery
Reaktivität: Human
7.428,00 €
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NEU
GPC3 - CHO-K1 Recombinant Cell Line
GPC3 - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78100

Recombinant CHO-K1 cells expressing GPC3 (Glypican 3), a cell surface proteoglycan bearing heparin-sulfate, ref. seq. NM_004484.2.
Schlagworte: OCI5, GPC3
Anwendung: Screening, Validating antibodies, CAR-T, Immunotherapy research, Drug discovery
Reaktivität: Human
7.428,00 €
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NEU
Human Mesothelin - CHO-K1 Recombinant Cell Line
Human Mesothelin - CHO-K1 Recombinant Cell Line

Artikelnummer: BPS-78132

Recombinant CHO-K1 cells expressing cleaved, mature human Mesothelin (MSLN) gene (GenBank Accession #NM_005823.5) under the control of cytomegalovirus (CMV) promoter. This cell line was generated by limited dilution and isolation of individual clones, which were screened based on MSLN expression to obtain a...
Schlagworte: MPF, MSLN
Anwendung: MSLN-expressing target cells, MSLN CAR-T cells co-culture assays, Screening
Reaktivität: Human
7.428,00 €
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NEU
Cas9-Expressing HCT116 Cell line - High expression
Cas9-Expressing HCT116 Cell line - High expression

Artikelnummer: BPS-78135-H

This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS-78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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Cas9-Expressing Jurkat Cell line - High expression
Cas9-Expressing Jurkat Cell line - High expression

Artikelnummer: BPS-78136-H

This cell line is a clonal derivative from the Cas9-Expressing Jurkat Cell Pool (BPS-78070). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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NEU
Cas9-Expressing Neuro2a Cell line - High expression
Cas9-Expressing Neuro2a Cell line - High expression

Artikelnummer: BPS-78137-H

This cell line is a clonal derivative from the Cas9-Expressing Neuro2a Cell Pool (BPS-78087). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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Human EGFRvIII- CHO K1 Recombinant Cell Line (High or Low Expression)-78145-H
Human EGFRvIII- CHO K1 Recombinant Cell Line (High or Low...

Artikelnummer: BPS-78145-H

Recombinant hEGFRvIII-CHO K1 cell line stably expressing full-length human EGFRvIII receptor (accession number NP_001333870). Surface expression of hEGFRvIII was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hEGFRvIII expression (High and Low) to mimic different...
Schlagworte: ERBB, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase erbB-1
Anwendung: EGFRvIII signaling inhibitor screening, Immuno-oncology research, Drug discovery
Reaktivität: Human
7.428,00 €
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CD38 / BCMA / Firefly Luciferase CHO Recombinant Cell Line
CD38 / BCMA / Firefly Luciferase CHO Recombinant Cell Line

Artikelnummer: BPS-78148

Recombinant clonal stable CHO cell line constitutively expressing full length human CD38 protein (also known as ADPRC1, Genbank accession #NM_001775) and human BCMA protein (B-Cell Maturation Antigen or CD269, GenBank accession #NM_001192). This cell line was derived from our CHO-K1 Luciferase cells (BPS-79725),...
Schlagworte: T10, CD38, ADPRC 1, EC=3.2.2.6, EC=2.4.99.20, cADPr hydrolase 1, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1,...
Anwendung: Validation
Reaktivität: Human
10.274,00 €
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NEU
CD38 / CD19 / Firefly Luciferase CHO Recombinant Cell Line
CD38 / CD19 / Firefly Luciferase CHO Recombinant Cell Line

Artikelnummer: BPS-78149

Recombinant clonal stable CHO cell line constitutively expressing full length human CD38 protein (also known as ADPRC1, Genbank accession #NM_001775) and human CD19 protein (also known as B4 or CVID3, Genbank accession #NM_001770). This cell line was derived from our CHO-K1 Luciferase cells (BPS-79725), therefore it...
Schlagworte: T10, CD38, ADPRC 1, EC=3.2.2.6, EC=2.4.99.20, cADPr hydrolase 1, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1,...
Anwendung: Validation
Reaktivität: Human
10.274,00 €
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NEU
Cas9-Expressing MDA-MB-231 Cell Line (High or Low Expression)-78150-H
Cas9-Expressing MDA-MB-231 Cell Line (High or Low...

Artikelnummer: BPS-78150-H

This cell line is a clonal derivative from the Cas9-Expressing MDA-MB-231 Cell Pool (BPS-78069). It was generated by limited limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low- or high-expressing cell line. The expressed Cas9...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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NEU
Cas9-Expressing Raji Cell Line
Cas9-Expressing Raji Cell Line

Artikelnummer: BPS-78156

This cell line is a clonal derivative from the Cas9-Expressing Raji Cell Pool (BPS-78071). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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NEU
Cas9-Expressing Daudi Cell Line
Cas9-Expressing Daudi Cell Line

Artikelnummer: BPS-78157

This cell line is a clonal derivative from the Cas9-Expressing Daudi Cell Pool (BPS-78089). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a...
Schlagworte: SpCas9, SpyCas9, SPy_1046, CRISPR-associated endonuclease Cas9/Csn1
Anwendung: Knock-out generation, SgRNA screen implementation
7.428,00 €
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