Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Artikelnummer: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Anwendung: RNA quantification
45,00 €
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TDO Primer Mix-qPCR Assay
TDO Primer Mix-qPCR Assay

Artikelnummer: BPS-71271

Primer mix for amplifying human TDO cDNA (1221 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Schlagworte: TO, TDO, TRPO, Tryptophanase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptamin 2,3-dioxygenase, Tryptophan...
Anwendung: RT-qPCR, RT-PCR
Reaktivität: Human
178,00 €
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IDO1 Primer Mix-qPCR Assay
IDO1 Primer Mix-qPCR Assay

Artikelnummer: BPS-71272

Primer mix for amplifying human IDO1 cDNA (1212 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Schlagworte: IDO, IDO1, IDO-1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase
Anwendung: RT-qPCR, RT-PCR
Reaktivität: Human
178,00 €
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Biotoxis qPCR Detection Kit
Biotoxis qPCR Detection Kit

Artikelnummer: Cay28322-1

Biotoxis is a qPCR all-in-one detection kit that detects B. anthracis, Y. pestis and F. tularensis. These 3 pathogens are the first potential biological weapon that can cause lethal infection. This kit is the only one on the market that detects in one reaction all 3 agents, allowing a rapid response in a context of...
Anwendung: Sensitive B. anthracis, Y. pestis and F. tularensis multiplex qPCR detection
2.500,00 €
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COVID-19 Forschung
COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit
COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit

Artikelnummer: G-VA0001.100

Research Use Only (RUO). This kit has not been tested and validated by any public health agency. This product is a multiplex fluorescent probe-based RT-qPCR assay system for the detection of COVID-19, the disease caused by the novel coronavirus SARS-CoV-2. The Taqman fluorescent probe is a specific oligonucleotide...
Schlagworte: SARS-CoV-2 Detection Kit - Triplex Taqman RT-qPCR Kit for Coronavirus Detection (2019-nCoV)
Anwendung: RT-qPCR
Reaktivität: Human
1.049,00 €
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Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Artikelnummer: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
111,00 €
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ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: ACTB, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Anwendung: RNA quantification
45,00 €
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Human Antigen Processing and Presentation Primer Library
Human Antigen Processing and Presentation Primer Library

Artikelnummer: HAPP-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against Antigen Processing and Presentation related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA...
Schlagworte: HAPP-I
Anwendung: Gene expression profiling, PCR array
687,00 €
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Human BDNF Signaling Primer Library
Human BDNF Signaling Primer Library

Artikelnummer: HBDNF-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against BDNF Signaling related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and SYBR green...
Schlagworte: HBDNF-I
Anwendung: Gene expression profiling, PCR array
687,00 €
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Human Oncogene circRNA Library
Human Oncogene circRNA Library

Artikelnummer: HCirc-Onc-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against Oncogene circRNA Library related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and...
Schlagworte: HCirc-Onc-I
Anwendung: Gene expression profiling, PCR array
1.242,00 €
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Human ERBB Signaling Primer Library
Human ERBB Signaling Primer Library

Artikelnummer: HERBB-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against ERBB Signaling related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and SYBR green...
Schlagworte: HERBB-I
Anwendung: Gene expression profiling, PCR array
687,00 €
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Human GDNF Signaling Primer Library
Human GDNF Signaling Primer Library

Artikelnummer: HGDNF-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against GDNF Signaling related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and SYBR green...
Schlagworte: HGDNF-I
Anwendung: Gene expression profiling, PCR array
687,00 €
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