Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen . In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die in der Regel aus 20 bis 40 Nukleotiden bestehen. In der Molekularbiologie finden Primer vor allem bei der PCR (Polymerase-Kettenreaktion) Verwendung, welche die in vitro-Vervielfältigung von DNA ermöglicht. Die Vervielfältigung wird durch DNA-Polymerasen erreicht, denen die Primer als Startpunkt bei der DNA-Synthese dienen. Primer erlauben die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qRT-PCR (quantitative Real-Time PCR), die quantitative Detektion von Nukleinsäuren.

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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Artikelnummer: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Anwendung: RNA quantification
45,00 €
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Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Artikelnummer: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
145,00 €
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ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: ACTB, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Anwendung: RNA quantification
45,00 €
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STI11 (Genital ulcer 2) (QuantStudio) Multiplexed qPCR Assay Bundle
STI11 (Genital ulcer 2) (QuantStudio) Multiplexed qPCR...

Artikelnummer: Cay41403-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Schlagworte: Sexually Transmitted Infection
Anwendung: Optimized for QuantStudio 5, 6, 7, 12K
814,00 €
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Bacterial Vaginosis Primary (QuantStudio) Multiplexed qPCR Assay
Bacterial Vaginosis Primary (QuantStudio) Multiplexed...

Artikelnummer: Cay41401-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram+, Multiplexed Reaction Panel:...
Anwendung: Optimized for QuantStudio 5, 6, 7, 12K
814,00 €
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Wound4 (Bio-Rad) Multiplexed qPCR Assay Bundle
Wound4 (Bio-Rad) Multiplexed qPCR Assay Bundle

Artikelnummer: Cay41488-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 3 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Bacteria Gram+, Multiplexed Reaction Panels: Wound Colors:...
Schlagworte: WND4
Anwendung: Optimized for Bio-Rad CFX96 / CFX384
814,00 €
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Candidiasis Primary (QuantStudio) Multiplexed qPCR Assay Bundle
Candidiasis Primary (QuantStudio) Multiplexed qPCR Assay...

Artikelnummer: Cay41402-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: 2X qPCR enzyme mastermix  Tube 4: Spike-in RPP30...
Schlagworte: Clavispora lusitaniae
Anwendung: Optimized for QuantStudio 5, 6, 7, 12K
814,00 €
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E.coli Residual Total RNA Detection Kit
E.coli Residual Total RNA Detection Kit

Artikelnummer: ARG83092.100

E.coli Residual Total RNA Detection Kit is designed for the quantitative detection of residual E.coli total RNA in various biological products to improve control quality of nucleic acid. This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR technology and fluorescent...
Anwendung: qPCR
2.630,00 €
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TDO Primer Mix-qPCR Assay
TDO Primer Mix-qPCR Assay

Artikelnummer: BPS-71271

Primer mix for amplifying human TDO cDNA (1221 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Schlagworte: TO, TDO, TRPO, Tryptophanase, Tryptophan pyrrolase, Tryptophan oxygenase, Tryptamin 2,3-dioxygenase, Tryptophan...
Anwendung: RT-qPCR, RT-PCR
Spezies-Reaktivität: human
257,00 €
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IDO1 Primer Mix-qPCR Assay
IDO1 Primer Mix-qPCR Assay

Artikelnummer: BPS-71272

Primer mix for amplifying human IDO1 cDNA (1212 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Schlagworte: IDO, IDO-1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase,
Anwendung: RT-qPCR, RT-PCR
Spezies-Reaktivität: human
257,00 €
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COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit
COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit

Artikelnummer: G-VA0001.100

This product is a multiplex fluorescent probe-based RT-qPCR assay system for the detection of COVID-19, the disease caused by the novel coronavirus SARS-CoV-2. The Taqman fluorescent probe is a specific oligonucleotide based on a reporter-quencher mechanism. For each probe, the 5'-end is labeled with a fluorophore,...
Schlagworte: SARS-CoV-2 Detection Kit - Triplex Taqman RT-qPCR Kit for Coronavirus Detection (2019-nCoV)
Anwendung: RT-qPCR
Spezies-Reaktivität: human
954,00 €
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E.coli Residual DNA Detection Kit
E.coli Residual DNA Detection Kit

Artikelnummer: ARG83091.100

E.coli Residual DNA Detection Kit is designed for the quantitative detection of E.coli host cell DNA in intermediates, semifinished products and finished products of various biological products. This kit adopts the principle of Taqman probe to quantitatively detect E.coli residual DNA in samples. The kit is a rapid,...
Anwendung: qPCR
2.047,00 €
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