Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

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Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Artikelnummer: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
111,00 €
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Mouse Housekeeping Genes Primer Sets
Mouse Housekeeping Genes Primer Sets

Artikelnummer: MHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, and RPL13A. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
97,00 €
Bewerten
Rat Housekeeping Genes Primer Sets
Rat Housekeeping Genes Primer Sets

Artikelnummer: RHK-1

Includes primer sets for Actb, B2m, Gapd, Gusb, Hprt1, Pgk, Pp1a, Rpl13a, Tbp and Tfrc. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
111,00 €
Bewerten
ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Artikelnummer: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
IL1B, Human interleukin 1, beta, Real Time PCR Primer Set
IL1B, Human interleukin 1, beta, Real Time PCR...

Artikelnummer: VHPS-4531

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
IL6, Human interleukin 6 (interferon, beta 2), Real Time PCR Primer Set
IL6, Human interleukin 6 (interferon, beta 2),...

Artikelnummer: VHPS-4559

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
IL8, Human interleukin 8, Real Time PCR Primer Set
IL8, Human interleukin 8, Real Time PCR Primer Set

Artikelnummer: VHPS-4563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
TNF, Human tumor necrosis factor (TNF superfamily, member 2), Real Time PCR Primer Set
TNF, Human tumor necrosis factor (TNF...

Artikelnummer: VHPS-9415

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
VEGF, Human, Real Time PCR Primer Set
VEGF, Human, Real Time PCR Primer Set

Artikelnummer: VHPS-9899

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
Il6, Mouse interleukin 6, Real Time PCR Primer Set
Il6, Mouse interleukin 6, Real Time PCR Primer Set

Artikelnummer: VMPS-3096

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
B2M, Mouse beta-2 microglobulin, Real Time PCR Primer Set
B2M, Mouse beta-2 microglobulin, Real Time PCR...

Artikelnummer: VMPS-563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended reaction conditions: primer concentration - 0.1 µM, MgCl2...
Anwendung: RNA quantification
Format: Solution
45,00 €
Bewerten
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