Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung,... mehr erfahren »
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Primer

Primer sind kurze Oligonukleotide, die Polymerasen als Startpunkt bei der DNA-Synthese dienen. In der Molekularbiologie finden Primer vor allem in der PCR (Polymerase-Kettenreaktion) Verwendung, bei der DNA in vitro vervielfältigt wird. Primer erlauben auch die spezifische Amplifikation von DNA-Abschnitten für Klonierungen oder, in Form der qPCR (Quantitative Real-Time PCR), die quantitative Detektion von Nulkleinsäuren.

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Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Artikelnummer: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
111,00 €
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Mouse Housekeeping Genes Primer Sets
Mouse Housekeeping Genes Primer Sets

Artikelnummer: MHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, and RPL13A. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
97,00 €
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Rat Housekeeping Genes Primer Sets
Rat Housekeeping Genes Primer Sets

Artikelnummer: RHK-1

Includes primer sets for Actb, B2m, Gapd, Gusb, Hprt1, Pgk, Pp1a, Rpl13a, Tbp and Tfrc. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Anwendung: RT-PCR, Gene expression analysis
111,00 €
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18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set
18S, Human 18S Ribosomal RNA gene, Real Time PCR Primer Set

Artikelnummer: VHPS-10350

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: qPCR, gene expression analysis
Anwendung: RNA quantification
45,00 €
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ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: ACTB, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Anwendung: RNA quantification
45,00 €
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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Artikelnummer: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Anwendung: RNA quantification
45,00 €
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IL1B, Human interleukin 1, beta, Real Time PCR Primer Set
IL1B, Human interleukin 1, beta, Real Time PCR Primer Set

Artikelnummer: VHPS-4531

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: IL1B, IL1F2, IL-1 beta, Catabolin, Interleukin-1 beta
Anwendung: RNA quantification
45,00 €
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IL6, Human interleukin 6 (interferon, beta 2), Real Time PCR Primer Set
IL6, Human interleukin 6 (interferon, beta 2), Real Time...

Artikelnummer: VHPS-4559

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: CDF, IL6, IL-6, BSF-2, IFNB2, IFN-beta-2, Interleukin-6, Interferon beta-2, Hybridoma growth factor, CTL differentiation...
Anwendung: RNA quantification
45,00 €
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IL8, Human interleukin 8, Real Time PCR Primer Set
IL8, Human interleukin 8, Real Time PCR Primer Set

Artikelnummer: VHPS-4563

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: NAF, IL8, IL-8, MDNCF, LYNAP, NAP-1, GCP-1, CXCL8, MONAP, MDNCF-a, MDNCF-b, MDNCF-c, Emoctakin, IL-8(5-77), IL-8(6-77),...
Anwendung: RNA quantification
45,00 €
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TNF, Human tumor necrosis factor (TNF superfamily, member 2), Real Time PCR Primer Set
TNF, Human tumor necrosis factor (TNF superfamily, member...

Artikelnummer: VHPS-9415

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: TNF, TNFA, TNFA protein, Tumor necrosis factor-alpha, Tumor necrosis factor (TNF superfamily, member 2), cDNA, FLJ95875,...
Anwendung: RNA quantification
45,00 €
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VEGF, Human, Real Time PCR Primer Set
VEGF, Human, Real Time PCR Primer Set

Artikelnummer: VHPS-9899

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: VPF, VEGF, VEGFA, VEGF-A, Vascular permeability factor, Vascular endothelial growth factor A
Anwendung: RNA quantification
45,00 €
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Il1B, Mouse interleukin 1 beta, Real Time PCR Primer Set
Il1B, Mouse interleukin 1 beta, Real Time PCR Primer Set

Artikelnummer: VMPS-3072

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Schlagworte: Il1b, IL-1 beta, Interleukin-1 beta
Anwendung: RNA quantification
45,00 €
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