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Leupeptin (Ac-Leu-Leu-Arginal-hemisulfate) Artikelnummer: 12136.25
Leupeptin, also known as N-acetyl-L-leucyl-L-leucyl-L-argininal, is a naturally occurring protease inhibitor that can inhibit cysteine, serine and threonine peptidases. It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases,...
| Anwendung: | Inhibitor |
| CAS | 103476-89-7 |
| MW: | 475.6 |
ab 83,00 €
Sodium Dodecyl Sulfate (SDS), pure Artikelnummer: 04051.1
SDS is commonly used in the preparation of proteins for electrophoresis in SDS-PAGE. This compound works by disrupting non-covalent bonds in proteins, denaturing them and causing the proteins to lose their natural conformation. The new negative charge is significantly larger than the original charge of that protein....
| Anwendung: | Anionic detergent |
| CAS | 151-21-3 |
| MW: | 288.38 |
42,00 €
Glycine, ultra pure Artikelnummer: 04943.1
Glycine (abbreviated as Gly or G) is the smallest of the 20 amino acids commonly found in proteins, having a hydrogen substituent as its side-chain. Its codons are GGU, GGC, GGA, GGG of the genetic code. Glycine is often used as a counterion in SDS-PAGE and in buffers for western blotting.
| Anwendung: | Buffer component, Protein stabilization |
| CAS | 56-40-6 |
| MW: | 75.07 |
ab 30,00 €
Isopropyl-beta-D-thiogalacto- pyranoside (IPTG) Artikelnummer: 05684.25
Purity: >99%. Free of dioxane and other solvents. For use im molecular biology. In combination with X-Gal (cat# 02249) it is used to detect lac+ colonies in cloning experiments.
| Anwendung: | Inducer of beta-galactosidase |
| CAS | 367-93-1 |
| MW: | 238.30 |
ab 32,00 €
MES, ultra pure Artikelnummer: 06010.100
MES (2-(N-morpholino)ethanesulfonic acid) is used as a buffering agent. It has pKa value of 6.15 at 20°C. The pH (and pKa at ionic strength I?0) of the buffer solution changes with concentration and temperature. MES is highly soluble in water. The melting point is approx. 300 °C. MOPS is a similar pH buffering...
| CAS | 4432-31-9 |
| MW: | 195.24 |
ab 61,00 €
Tris, ultra pure Artikelnummer: 08003.1
Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It is a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes.
| Schlagworte: | Trometamol, Tromethamin, Tris(hydroxymethyl)-aminomethan, 2-Amino-2-(hydroxymethyl)-propan-1,3-diol |
| CAS | 77-86-1 |
| MW: | 121.14 |
ab 61,00 €
Dimethylsulfoxide (DMSO) Artikelnummer: 54686.100
DMSO is a polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. DMSO is less toxic than other members of this class, such as dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and HMPA. DMSO is also used in PCR to...
| Anwendung: | Protein stabilization |
| CAS | 67-68-5 |
| MW: | 78.13 |
ab 29,00 €
HEPES, sodium salt Artikelnummer: 50432.1
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by...
| CAS | 75277-39-3 |
| MW: | 260.28 |
ab 54,00 €
MOPS, sodium salt Artikelnummer: 50429.1
Useful pH range: 6.5-7.9
| CAS | 71119-22-7 |
| MW: | 231.25 |
ab 62,00 €
Tricine, ultra pure Artikelnummer: 22870.1
Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be...
| CAS | 5704-04-1 |
| MW: | 179.17 |
ab 46,00 €
MOPS, ultra pure Artikelnummer: 06170.1
MOPS is frequently used as a buffering agent. It is a structural analog to MES. Its chemical structure contains a morpholine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
| CAS | 1132-61-2 |
| MW: | 209.26 |
ab 32,00 €
HEPES, ultra pure Artikelnummer: 05288.1
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by...
| CAS | 7365-45-9 |
| MW: | 238.31 |
ab 24,90 €