6 products were found matching "Q60876"!

4E-BP1 (Phospho-Thr69) Colorimetric Cell-Based ELISA Kit Protein function: Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

When bound to eIF4E, 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are...

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR...

Source:, Recombinant Mouse from E. coli, Purity: >95%, Endotoxin: 1.0EU per 1ug (determined by the LAL method), Accession No: Q60876, Fragment: Ser2~Ile117 (Accession No: Q60876), Sequence: MGHHHHHHSGSEF-SAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS...

Sample Type:, Cell line of choice, Intended Use: The Phospho-4E-BP1 (Thr69) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-4E-BP1(Thr69) in plated and fixed cells. Sensitivity: >5000cells/well, Assay Time: 4.5 hours, Reactivity: Mouse:T69, Rat:T69, Detection Method: Colorimetric 450...