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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
G-MORV0006.10 | 10 rxns | - | - |
9 - 20 business days* |
341.00€
|
||
G-MORV0006.25 | 25 rxns | - | - |
9 - 20 business days* |
716.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
GenieClone Site-Directed Mutagenesis Kit v2 is designed for rapid site-directed mutagenesis of 3... more
Product information "GenieClone Site-Directed Mutagenesis Kit v2"
GenieClone Site-Directed Mutagenesis Kit v2 is designed for rapid site-directed mutagenesis of 3 to 5 separate mutations in the same reaction in less than 3 hours. Based on GenieClone rapid cloning technology, this kit uses homologous recombination to replace the conventional annealing ring-forming reactions meaning much less template and a more flexible primer design strategy. With seamless splicing of the two PCR products by GenieClone technology, the kit can complete up to two separate mutations with a single amplification reaction. DpnI digested products of the specific amplicons can be directly added to the recombination reaction without purification. The highly optimized reaction buffer, fast protocol and superior site-directed mutagenesis efficiency make GenieClone Site-Directed Mutagenesis Kit v1 make an excellent choice for introducing 3 to 5 mutations at discontinuous sites simultaneously on the same plasmid. Key Features: * Introduce 3 - 5 separate mutations in the same reaction. * Site-directed mutagenesis can be performed on up to 5 discontinuous sites (more than 50 bp apart) simultaneously on the target plasmid. * Single kit for cloning and mutagenesis. * Includes Genie Fusion Ultra High-Fidelity DNA Polymerase providing high-fidelity PCR with extremely low mutation rates & excellent long fragment amplification for any plasmid amplification up to 20 kb as well as GC-rich templates. * Amplification is carried out exponentially and the template usage is extremely low, which is beneficial to the complete degradation of the original methylation template. * DpnI eliminates contamination of the original template and decreases background. * Amplified products can be directly used in the recombination reaction after DpnI treatment. * The reaction mixture can be used to directly transform chemically competent E.coli cells.
Supplier: | Assay Genie |
Supplier-Nr: | MORV0006 |
Properties
Application: | 3 to 5 separate mutations |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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