BPP Bioportide(TM) Fungal Transformation Kit for Ascomycota

BPP Bioportides(TM) are novel multi-functional designer proteins that bind quickly, spontaneously, reversibly, and robustly nucleic acids and transport them across the host's cell membrane utilizing a specialized N-terminal cell-penetrating-peptide moiety. This domain is designed differently for each BPP Bioportide(TM), depending on which type of host cell is to be addressed. The BPP Bioportide(TM) Transformation Kit for Ascomycota provides a ready-to-use system for efficient and fast transformation of Ascomycota especially for transforming strains that have not previously taken up foreign nucleic acids or have done so only with great difficulty. The BPP Bioportide(TM) enables the transport of nucleic acids, ranging from 22 nt to >10 kb, into cells. Both RNA and DNA, including single- and double-stranded, linear or circular forms, can be transferred reliably. This innovative reagent facilitates the uptake of nucleic acids without requiring inducing cell stress, i.e. heat shock, or competent cells, i.e. by means of electroporation or means of bacterial conjugation. The easy and fast protocol works with nanogram quantities of nucleic acids. BPP Bioportides(TM) show robust performance in extreme environments (up to 80°C, pH > 9.0, high salt concentrations).