Peroxiredoxin I, active human recombinant protein

Active human Peroxiredoxin I (GenBank Accession No. NM_005614), full length, with N-terminal HIS6 tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system. 1.88 µg of PRDX1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30 °C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.