Feruloyl esterase 1A, Clostridium thermocellum

Feruloyl esterase 1A (CtFae1A), assigned the E.C. number 3.1.1.73, is a derivative of Clostridium thermocellum. It is an enzyme that hydrolyzes the ferulate groups involved in the crosslinking of hemicelluloses to lignin. The recombinant CtFae1A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 1 (CE1) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 2 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Feruloyl esterases. Family: CE1. Ec Number: 3.1.1.73. Organism: Clostridium thermocellum. Architecture: CE1. Optimal pH: 6. Optimal Temperature: 60°C. Substrates: FAX3, FAXX, PAX3