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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E2240-20.150 | 150 µl | - | - |
3 - 19 business days* |
369.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The transcription factor Elk-1 is a component of the ternary complex that binds the serum... more
Product information "Elk-1 Control Proteins,"
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus, phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase, (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity, (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). Molecular Weight: 40kD nonphosphorylated, 50kD phosphorylated, Nonphosphorylated Elk1 fusion protein: Bacterially expressed GST-Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20°C. Phosphorylated Elk1 fusion protein: GST-Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20°C. Directions for Use: As controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading. 150ul wil be sufficient for 10 blots. Western Blots: For controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.
Supplier: | United States Biological |
Supplier-Nr: | E2240-20 |
Properties
Conjugate: | No |
Database Information
KEGG ID : | K04375 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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