Mca-EVKMDAEF-K(Dnp)-NH2 (ammonium salt)

Mca-EVKMDAEF-K(Dnp)-NH2 is a fluorogenic substrate for beta-secretase. Upon cleavage by beta-secretase, 7-methoxycoumarin-4-acetyl (Mca) is released and its fluorescence can be used to quantify beta-secretase activity. Mca displays excitation/emission maxima of 328/420 nm, respectively.Formal Name: 4S-(((S)-1-(((S)-1-amino-6-((2,4-dinitrophenyl)amino)-1-oxohexan-2-yl)amino)-1-oxo-3-phenylpropan-2-yl)carbamoyl)-16S-(4-aminobutyl)-10S-(carboxymethyl)-19S-isopropyl-22S-(2-(7-methoxy-2-oxo-2H-chromen-4-yl)acetamido)-7S-methyl-13S-(2-(methylthio)ethyl)-6,9,12,15,18,21-hexaoxo-5,8,11,14,17,20-hexaazapentacosanedioic acid, ammonium salt. Synonyms: beta-Secretase Fluorogenic Substrate. Molecular Formula: C66H88N14O23S . XNH3. Formula Weight: 1477.6. Purity: >95%. Formulation: (Request formulation change), A lyophilized powder. Solubility: 5% NH3 (Aqueous): 1 mg/ml. SMILES: NC([C@@H](NC([C@H](CC1=CC=CC=C1)NC([C@@H](NC([C@H](C)NC([C@H](CC(O)=O)NC([C@H](CCSC)NC([C@@H](NC([C@@H](NC([C@@H](NC(CC(C(C=CC(OC)=C2)=C2O3)=CC3=O)=O)CCC(O)=O)=O)C(C)C)=O)CCCCN)=O)=O)=O)=O)CCC(O)=O)=O)=O)CCCCNC4=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C4)=O.N. InChi Code: InChI=1S/C66H88N14O23S.H3N/c1-35(2)57(78-63(94)45(21-23-53(82)83)71-52(81)30-38-31-56(88)103-51-33-40(102-4)18-19-41(38)51)66(97)75-44(16-9-11-26-67)60(91)74-47(25-28-104-5)62(93)77-49(34-55(86)87)64(95)70-36(3)59(90)73-46(22-24-54(84)85)61(92)76-48(29-37-13-7-6-8-14-37)65(96)72-43(58(68)89)15-10-12-27-69-42-20-17-39(79(98)99)32-50(42)80(100)101,/h6-8,13-14,17-20,31-33,35-36,43-49,57,69H,9-12,15-16,21-30,34,67H2,1-5H3,(H2,68,89)(H,70,95)(H,71,81)(H,72,96)(H,73,90)(H,74,91)(H,75,97)(H,76,92)(H,77,93)(H,78,94)(H,82,83)(H,84,85)(H,86,87),1H3/t36-,43-,44-,45-,46-,47-,48-,49-,57-,/m0./s1. InChi Key: QLHLBDVKXDKTNS-IDRHJCDISA-N.