Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt)

Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage. Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 400/505 nm.Formal Name: (3S,6S,9S,12S,15S)-3-acetamido-19-amino-6,9,12-tris(carboxymethyl)-4,7,10,13-tetraoxo-15-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-5,8,11,14-tetraazanonadecanoic acid, trifluoroacetate salt. Molecular Formula: C34H40F3N7O16 . XCF3COOH. Formula Weight: 859.7. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 10 mg/ml, PBS (pH 7.2): 2.5 mg/ml. lambdamax: 234, 340 nm. SMILES: NCCCC[C@H](NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC(C)=O)=O)=O)=O)=O)C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C34H40F3N7O16.C2HF3O2/c1-14(45)39-19(10-24(46)47)30(56)42-21(12-26(50)51)32(58)44-22(13-27(52)53)33(59)43-20(11-25(48)49)31(57)41-18(4-2-3-7-38)29(55)40-15-5-6-16-17(34(35,36)37)9-28(54)60-23(16)8-15,3-2(4,5)1(6)7/h5-6,8-9,18-22H,2-4,7,10-13,38H2,1H3,(H,39,45)(H,40,55)(H,41,57)(H,42,56)(H,43,59)(H,44,58)(H,46,47)(H,48,49)(H,50,51)(H,52,53),(H,6,7)/t18-,19-,20-,21-,22-,/m0./s1. InChi Key: JKYYEGXSJPEGSE-QXJGFYJDSA-N.