Arginine, dimethyl, Antibody Sampler Pack

Kit Components:, A3550-06A Arginine, symmetric, dimethyl (SYM10) 1x25ul (Human, mouse) (WB, IP, ICC), Serum , Supplied as a liquid in 0.05% sodium azide, 30% glycerol., Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G (which R* denotes symmetric dimethylation of arginine) (KLH). A3550-06B Arginine, symmetric, dimethyl (SYM11) 1x25ug (Human) (WB) Purified by Protein A affinity chromatography. , Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide), 30% glycerol. , Concentration: 1mg/ml, Synthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH)., *When using A3550-06B as primary antibody, add 0.05% Twenn-20 to blocking buffer in steps 2. 3 and 5. A3550-06C Arginine, symmetric, dimethyl (ASYM24) 1x25ul (Human, mouse) (WB, IP, ICC) Serum , Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol., Synthetic peptide corresponding to KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G (which R* denotes asymmetric dimethylation of arginine) (KLH). Positive Control (Included): J9000 Jurkat Cell Lysate. 1x100ul Supplied as a liquid in modified RIPA buffer diluted with non-reducing sample buffer. , Concentration: 1mg/ml, Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: A3550-06A: 1:500-1:1000, A3550-06B: 1-2ug/ml , A3550-06C: 1:500-1:1000 , Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Antibody aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.