Substrates and Inhibitors

Solid lyophilized powder. Soluble in DMSO. Fluorogenic substrate for the peptidylglutamyl-peptide hydrolysing (PGPH) (caspase-like) activity of the 20S proteasome, Excitation: 380nm. Emission: 460nm.

Calpain inhibitor I is a synthetic tripeptide aldehyde that acts as a potent inhibitor of cysteine proteases including calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 500 pM). At 10-100 µM calpain inhibitor I dose-dependently prevents the degradation of...

Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the beta5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to...

A fluorogenic substrate for HDACs, AMC is released upon enzymatic cleavage by HDACs and its fluorescence can be used to quantify HDAC activity, ex/em = 340-360/440-460 nm, respectively. Formulation: A solid. InChI:...

A fluorogenic caspase-8/granzyme B substrate containing the acetyl (Ac) moiety.

Caspase 9 is a member of the CED-3 subfamily of the caspase family of cysteine proteases that play an essential role in the execution phase of apoptosis. These enzymes share a dominant primary specificity for cleaving bonds following aspartic acid residues. Initiator caspases (such as caspase 8) activate effector...

Lyophilized powder. Soluble in DMSO. Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the beta5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at...

FITC-C6-DEVD-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. FITC-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.

Ac-LETD-AFC is a fluorogenic substrate that can be cleaved specifically by caspase-8. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name:...

Calpain inhibitor II is a cell permeable, peptide aldehyde inhibitor of calpain I (Ki = 120 nM), calpain II (Ki = 230 nM), cathepsin B (Ki = 100 nM), and cathepsin L (Ki = 0.6 nM). It has been used to demonstrate the involvement of ubiquitin-proteasome protein degradation in various biological systems.Formal Name:...

Ac-LEVD-CHO is a caspase-4 inhibitor. It inhibits IL-1alpha expression and secretion and caspase-4 activation induced by the T. denticola periodontal pathogen surface protein Td92 in human gingival fibroblasts when used at a concentration of 30 µM. Ac-LEVD-CHO (10 µM) also reduces apoptosis induced by expression of...

FAM-VAD is a green fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently...