Substrates and Inhibitors

The non-fluorescent R110 substrates generate the bright green fluorescent rhodamine 110 product that has Ex/Em = 494/521 nm, and can be easily detected with a FITC filter set. In general, R110 substrates are much more sensitive than the AMC-, AFC- or 4-nitroaniline-based substrates. This R110 substrate is used for...

Solid lyophilized powder. Soluble in DMSO. Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases, Extinction: 380nm. Emission: 460 nm, This substrate is useful for inhibitor screening and kinetic analysis.

White to off-white solid. Soluble in water, ethanol, methanol, DMSO or acetonitrile. Potent reversible inhibitor of serine/cysteine proteases and some trypsin-like proteases. Inhibits papain, trypsin and trypsin-like serine proteases as well as cathepsin A, B and D, Similar activity spectrum comparable to leupeptin...

During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD. Ac-DEVD-AFC is a fluorogenic substrate for activated caspase-3 (Km = 9.7 µM), as well as related caspases. Caspase activity can be quantified by...

A fluorogenic substrate for HDACs, AMC is released upon enzymatic cleavage by HDACs and its fluorescence can be used to quantify HDAC activity, ex/em = 340-360/440-460 nm, respectively. Formulation: A solid. InChI:...

NFAT inhibitor is a peptide inhibitor of the transcription factor nuclear factor of activated T cells (NFAT). It binds to the NFAT recognition site on the protein phosphatase calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity when used at a concentration of 100 µM. NFAT inhibitor...

Ac-DEVD-AMC is a fluorogenic substrate that can be cleaved specifically by caspase-3. Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.Formal Name:...

A fluorogenic substrate for caspase 3. The 7-amido-4-trifluoromethylcoumarin derivatives have better membrane permeability than the 7-amido-4-methylcoumarin derivatives.

Ac-DEVD-CMK is a cell-permeable, and irreversible inhibitor of caspase-3 as well as caspase-6, -7, -8, and -10. It is commonly used at concentrations up to 100 µM to examine the role of caspase-3-dependent apoptosis in biological systems.Formal Name:...

Protein kinase C? (PKC?) is a calcium-independent, phospholipid- and diacylglycerol-dependent serine/threonine kinase involved in diverse signaling pathways, including those involved in neuronal signaling, cytoskeletal function, and inflammation. PKC? inhibitor peptide is a synthetic peptide corresponding to an...

Cleavage of R110 peptides by caspases generates strongly fluorescent R110 that can be monitored fluorimetrically at 510-530 nm with excitation of 488 nm.This R110 substrate is specific for caspases 3 and 7.

Caspofungin acetate is a lipopeptide antifungal that inhibits the enzyme 1,3-beta-Glucan synthase, which synthesizes -glucan polymers that form fungal cell walls. Caspofungin acetate is active against a variety of Aspergillus and Candida species and unlike many antifungals, is not metabolized by the cytochrome p450...