Retinoic Acid Receptor alpha, Control Peptide (RAR alpha, RARa)

Synthetic peptide corresponding to aa 10-25 of human RARa (retinoic acid receptor-alpha). (tpggghlngypvppya) (linear), Corresponding antibody R1691 is specific for Retinoic acid receptor, alpha isotype (~48kD). Species crossreactivity: Human and rat RAR alpha. , , Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition (1). While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrettís tissues compared with normal tissue (3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot (rat brain hippocampal homogenate): 1:1000. TBST (20 mM Tris/HCl (pH 7.6), 137 mM NaCl, 1% Tween-20) 5% nonfat milk, 0.05% sodium azide. A ~48kD band can be seen in a Western blot analysis, using rat brain hippocampal homogenate. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.