Neuropeptide Y5 Receptor, Mouse, Control Peptide (NPY 5R)

Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells. It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. NPY a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a mol. wt of 11kD. NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in amino acid and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop. NPY5R, a 466aa in mouse, 456aa in rat and 455 in human (chr 4q31-q32), it is the receptor for NPY and PYY. The activity of this receptor is mediated by G proteins that inhibit Adenylate cyclase activity. Expressed in brain and hypothalamus. Source: 15aa peptide from mouse NPY5R (1). Location: ~N-terminus , Extracellular domain, Species Sequence Homology: Rat-93%. No significant homology with human NPY5R. Applications: Suitable for use in ELISA as a control peptide for N2176-66. Other applications not tested. Recommended Dilution: ELISA: Coat ELISA plates at 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.