Neuropeptide EI (rat) (trifluoroacetate salt)

Neuropeptide EI (NEI) is an endogenous peptide fragment of rat prepro-melanin concentrating hormone (MCH) that is involved in hormone release, grooming behavior, and motor activity. NEI corresponds to amino acids 140-152 of rat prepro-MCH and 131-143 of human, mouse, and rat prepro-MCH. It is expressed in both the central and peripheral nervous systems, localizes to the cytoplasm, and colocalizes with MCH in the CNS. NEI (1 µM) reduces thyrotropin-releasing hormone (TRH) release in mediobasal hypothalamus explants. Intracerebroventricular administration of NEI increases serum levels of luteinizing hormone (LH) in male rats and in chronically ovariectomized female rats that received estradiol benzoate and progesterone in addition to NEI. NEI (1 µg/animal, i.c.v.) induces excessive grooming behavior and increases locomotor activity in rats, effects that can be blocked by MCH (Cay-24462) or alpha-melanocyte-stimulating hormone (alpha-MSH, Cay-29923). It also increases the amount of time spent in REM sleep in rats when injected intracerebroventricularly or into the ventrolateral periaqueductal grey (vlPAG).Formal Name: L-alpha-glutamyl-L-isoleucylglycyl-L-alpha-aspartyl-L-alpha-glutamyl-L-alpha-glutamyl-L-asparaginyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-L-prolyl-L-isoleucinamide, trifluoroacetate salt. Synonyms: NEI, Neuropeptide-Glutamic Acid-Isoleucine. Molecular Formula: C63H98N16O23 . XCF3COOH. Formula Weight: 1447.6. Purity: >95%. Formulation: (Request formulation change), A solid. Peptide Sequence: EIGDEENSAKFPI-NH2. Solubility: DMSO: Sparingly soluble: 1-10 mg/ml, PBS (pH 7.2): Soluble: >10 mg/ml. SMILES: OC(C(F)(F)F)=O.[H]N[C@@H](CCC(O)=O)C(N[C@]([C@H](CC)C)([H])C(NCC(N[C@H](C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](CC(N)=O)C(N[C@@H](CO)C(N[C@H](C(N[C@@H](CCCCN)C(N[C@H](C(N1CCC[C@H]1C(N[C@]([C@H](CC)C)([H])C(N)=O)=O)=O)CC2=CC=CC=C2)=O)=O)C)=O)=O)=O)=O)=O)CC(O)=O)=O)=O)=O. InChi Code: InChI=1S/C63H98N16O23.C2HF3O2/c1-6-31(3)50(52(67)91)77-61(100)43-17-13-25-79(43)63(102)41(26-34-14-9-8-10-15-34)75-55(94)36(16-11-12-24-64)71-53(92)33(5)69-60(99)42(30-80)76-58(97)39(27-44(66)81)74-57(96)38(20-23-48(87)88)72-56(95)37(19-22-47(85)86)73-59(98)40(28-49(89)90)70-45(82)29-68-62(101)51(32(4)7-2)78-54(93)35(65)18-21-46(83)84,3-2(4,5)1(6)7/h8-10,14-15,31-33,35-43,50-51,80H,6-7,11-13,16-30,64-65H2,1-5H3,(H2,66,81)(H2,67,91)(H,68,101)(H,69,99)(H,70,82)(H,71,92)(H,72,95)(H,73,98)(H,74,96)(H,75,94)(H,76,97)(H,77,100)(H,78,93)(H,83,84)(H,85,86)(H,87,88)(H,89,90),(H,6,7)/t31-,32-,33-,35-,36-,37-,38-,39-,40-,41-,42-,43-,50-,51-,/m0./s1. InChi Key: FABCGLFXCFXXIX-VXNMOIOASA-N.