Neudesin, Recombinant, Human, aa32-172 (CIR2, NENF, SCIRP10, SPUF, Neuron-derived Neurotrophic Secre

Neudesin (neuron­derived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalization­related 2) or GIG47, is a secreted, 20­21kD member of the MAPR (membrane­associated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141aa mature region that possesses a cytochrome b5­like heme­binding domain over aa44­129, and a lysine acetylation site at aa136 (1­4). The attachment of hemin to its heme­binding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5­6kD of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin over­expression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias, transfection studies indicate that it may act as an oncogene (4, 7). Source: Recombinant protein corresponding to aa32-172, Gly32-Phe172, from human Neudesin, fused at 6xHis-Tag at C-terminal, CHO-derived. Molecular Weight: ~19-21kD, Endotoxin: <0.1EU/1ug (LAL method), Biological Activity: Measured in a cell proliferation assay using Neuro?2A mouse neuroblastoma cells. The ED50 for this effect is 1.5-6.0ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.