Thioredoxin 1 Blocking Peptide

Peptide is used to block Thioredoxin 1 Rabbit mAb reactivity in immunohistochemistry protocols. Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5). , Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Thioredoxin 1 Rabbit mAb signal in immunohistochemistry. , , Directions For Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.