TACE, CT, Blocking Peptide (TNF-alpha Converting Enzyme ), Purified

Peptide corresponding to amino acids 807 to 823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid (3)., Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). , , TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association (6). Application: Used for blocking the activity of anti-TACE, catalog# T0500. Incubate the peptide with an equal volume of antibody for 30min. at 37°C usually completely blocks the activity of anti-TACE in Western Blotting.