RIP3, Blocking Peptide (Receptor Interacting Protein 3), Purified

Peptide (AQFGRGRGWQPFHK) corresponding to amino acids 473 to 486 of murine RIP3 (3). Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3). , SOURCE: Rabbit anti-RIP3 polyclonal antibody was raised against a peptide (AQFGRGRGWQPFHK) corresponding to amino acids 473 to 486 of murine RIP3 (3)., Applications: , Suitable for use in ELISA, Western Blotting. Other applications not tested. Positive Control: 3T3 cell lysate , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.