Recombinant Red Fluorescent Protein (RFP) Control

Item number Size Datasheet Manual SDS Delivery time Quantity Price
000-001-379 100 µg - -

3 - 9 business days*

453.00€
 
Recombinant RFP was synthesized with an amino terimal 6XHIS tag and was expressed in E.coli. A 3... more
Product information "Recombinant Red Fluorescent Protein (RFP) Control"
Recombinant RFP was synthesized with an amino terimal 6XHIS tag and was expressed in E.coli. A 3 step process was used to purify the protein: a partioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 95% pure. Recombinant RFP (wild type, sequence corresponding to RFP, Discosoma spp.) is intended for use as a control when using polyclonal or monoclonal anti-RFP in immunological assays, in fluorometry, and in fluorescence activated cell sorting (FACS). Polyclonal anti-RFP can detect RFP by ELISA and western blot. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA is well suited to titrate RFP in solution when used in combination with unconjugated anti-RFP antibody. This product can be used as a control in western blots.
Supplier: Rockland Immunochemicals
Supplier-Nr: 000-001-379

Properties

Conjugate: No

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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