DC-SIGN, CT, Blocking Peptide (Dendritic Cells, CD209)

Control Peptide for D1550, Synthetic peptide (CSRDEEQFLSPAPATPN PPPA) corresponding to aa384-404 of human DC-SIGN, C-terminal. Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. Application: , Suitable for use in ELISA And Antibody Blocking. Recommended Dilution: ELISA, Antibody Blocking: Incubating the peptide with an equal volume of antibody for 30 min at 37°C Optimal dilutions to be determined by the researcher., , Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.