Acinus, Blocking Peptide, Purified

Acinus (CT) peptide (SRSTPVRDRGGRR) corresponding to amino acids 1329 to 1341 of human AcinusL, 602 to 614 of human AcinusS', or 571 to 583 of human AcinusS, which are identical to those of mouse Acinus (2). For use with cat. no. A0600-01. Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS' (2). , Applications: , Suitable for use in Western Blot. Blocks the antibody activity completely when incubating peptide with equal volume of antibody for 30 min at 37ºC., ,