Tumor Necrosis Factor alpha (TNFa) Recombinant (Mouse)

Recombinant mouse tumor necrosis factor alpha (TNF-a), encompassing amino acids 80-235 (full length of TNF soluble form). This construct is tag-free. This protein was affinity purified. Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8+ cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.