Tumor Necrosis Factor, active (Tnf), Recombinant, Mouse

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation., The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {ECO:0000250}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1ng/ml, corresponding to a specific activity of ? 1.0x10e7 IU/mg in the presence of actinomycin D. Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL, Molecular Weight: 17.4kD, PubMed ID: 2836146, 3898078, 2419912, 2989794, 3040015, 3684584, 7560085, 9089109, 14656967, 2777790, 2268312, 3349526, 10089307, Gene Name: Tnf,Tnfa,Tnfsf2, Swiss Prot: P06804, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.