TGFB3, Recombinant, Human (Transforming Growth Factor beta-3, TGF-beta-3, FLJ16571)

The three mammalian isoforms of TGF-beta, TGF-beta1, beta2, beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-beta3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes. Recombinant human TGF-beta3 is a 25kD protein composed of two identical 112aa polypeptide chains linked by a single disulfide bond. Source: Recombinant protein corresponding to human TGF beta 3, expressed in E.coli. Biological Activity: Determined by TGF-B3's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be 0.05ng/ml , Specific Activity: 2x10e7units/mg, Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.