SDF-1-alpha, Recombinant, Human (CXCL12, Stromal Cell-derived Factor 1, SDF-1, hSDF-1, C-X-C Motif C

SDF-1 alpha and beta are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1alpha and beta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. Recombinant human SDF-1alpha is an 8kD protein containing 68aa. Source: Recombinant protein corresponding to human SDF-1 alpha, expressed in E.coli. Biological Activity: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml. Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.